@article { author = {S. A., Al-Mikhlafi and A.M, Othman}, title = {SYNTHESIS AND STABILITY STUDY ON AN ASPIRIN DERIVATIVE WITH POTENTIAL SELECTIVE COX-2 INHIBITOR}, journal = {Biochemistry Letters}, volume = {4}, number = {1}, pages = {1-14}, year = {2008}, publisher = {Zagazig University; Faculty of Science. Center of Scientific Studies and Researches}, issn = {1687-4773}, eissn = {2974-4725}, doi = {10.21608/blj.2008.64445}, abstract = {       The synthesis of N– [5– Methyl– 2– (1, 3, 4 COX-2 selective inhibitor and the preliminary kinetic study for hydrolysis of this compound is studied in this paper. The compound was prepared from the corresponding aspirin and 2–amino–5–methyl–1,3,4–thiadiazole in the presence of N,N-dicyclohexylcarbodiimide (DCC) as coupling agent .        The preliminary kinetic study for hydrolysis of this compound and the effect of pH on the rate of hydrolysis in buffer solution citrate–phosphate–borate/HCl was studied. The structure was confirmed by infrared, nuclear magnetic resonance (H–NMR), and elemental microanalysis assay. The compound underwent slightly hydrolysis at low and high pH with a fairly stabile so, provide a chance for it to be absorbed intact with highly bioavailability, longer half life, and with lower G1 toxicity.}, keywords = {}, url = {https://blj.journals.ekb.eg/article_64445.html}, eprint = {https://blj.journals.ekb.eg/article_64445_1240a4450e0ec5938c1dd2c7905047cd.pdf} } @article { author = {Gh., Osman, H. and G. E, Shiha, and K.A., El-Deeb,}, title = {EVALUATION OF ALPHA-GLUTATHIONE-S-TRANSFERASE AS A MARKER OF HEPATOCELLULAR DAMAGE}, journal = {Biochemistry Letters}, volume = {4}, number = {1}, pages = {15-40}, year = {2008}, publisher = {Zagazig University; Faculty of Science. Center of Scientific Studies and Researches}, issn = {1687-4773}, eissn = {2974-4725}, doi = {10.21608/blj.2008.64446}, abstract = {Alpha-glutathione-S-transferase (a-GST) are the cytoplasmatic class of enzymes responsible for cellular detoxifying processes. The serum a-GST activity in relation to chronic infection caused by hepatitis C virus (HCV) and acute infection caused by hepatitis A, B and C viruses. 15 anti-HCV negative and negative for HBV and HAV healthy subjects served (controls), 35 patients with chronic hepatitis [8/35 are anti-HCV positive subjects with normal alanine aminotransferases (ALT) and 27/35 chronic hepatitis are with high ALT levels]; 17 patients with acute liver disease (1 acute HCV infection, 3 acute HBV infections and 13 acute HAV infections were studied. Serum (a-GST) alpha glutathione-S-transferase, (aspartate aminotransferase, alkaline phosphatase (ALP), gamma glutamyl transferase GGT, total protein, albumin, total and direct bilirubin were assayed in all subjects, and after 0 week, 2 weeks and 4 weeks in the acute group.}, keywords = {}, url = {https://blj.journals.ekb.eg/article_64446.html}, eprint = {https://blj.journals.ekb.eg/article_64446_09d6a2533ae8fe59eac1b72809ef80d0.pdf} } @article { author = {F.M., Zahran and I ., EL-Dosoky and G.E., Shiha and A.T., Keshta and M.M., Omran and A.M., Attallah}, title = {DETECTION OF FIBRONECTIN IN SERA OF HCV INFECTED PATIENTS WITH LIVER FIBROSIS}, journal = {Biochemistry Letters}, volume = {4}, number = {1}, pages = {38-54}, year = {2008}, publisher = {Zagazig University; Faculty of Science. Center of Scientific Studies and Researches}, issn = {1687-4773}, eissn = {2974-4725}, doi = {10.21608/blj.2008.64447}, abstract = {An accurate assessment of liver fibrosis is very important in order to predict the prognosis and to start the appropriate therapy to prevent disease progression. Several biochemical markers have been proposed as indicators of the change from chronic hepatitis to cirrhosis. This study was carried out on 126 serum samples pathologically divided into two groups. A, the fibrotic group with different stages (F1-F3) consists of 114 patients (85 males and 29 females) with liver fibrosis associated with HCV infections (aged 50.6 ± 11.7 yrs.). B, the control group consists of 12 non-fibrotic (F0) individuals with HCV infection without fibrosis (aged 29.2 ± 5.2 yrs.), used as controls. The results revealed that the level of serum fibronectin increased in the all patients in the fibrotic group. Significant differences (p < 0.0001) were shown between non-fibrotic liver (F0), liver fibrosis (F1-F3). Fibronectin was positively correlated with age, ALT, AST, and bilirubin and negatively correlated with albumin. In conclusion, fibronectin fragment had been identified in serum a sample of fibrotic group at 90-kDa and its level was increased in these samples; while it was normal in non-fibrotic group. These data confirm the role of fibronectin as a pacemaker of fibrosis.}, keywords = {}, url = {https://blj.journals.ekb.eg/article_64447.html}, eprint = {https://blj.journals.ekb.eg/article_64447_e7d1400e2b126de4328df8c37d4d6aa8.pdf} } @article { author = {H.S., Hegazy, and S.M, Ghazi, and H.E., Daif,}, title = {STUDIES ON THE EFFECT OF ULTRASONIC WAVES ON: SUGAR METABOLISM AND SOME ACTIVITIES OF HYDROLYTIC ENZYMES OF ARACHIS HYPOGAEA SEEDLINGS AND CULTURED TISSUES}, journal = {Biochemistry Letters}, volume = {4}, number = {1}, pages = {55-68}, year = {2008}, publisher = {Zagazig University; Faculty of Science. Center of Scientific Studies and Researches}, issn = {1687-4773}, eissn = {2974-4725}, doi = {10.21608/blj.2008.64448}, abstract = {The variation of sugar metabolism and related enzymes of peanut seedlings and cultured tissues by ultrasonic waves were carried out in this work. It was found that reducing sugars and sucrose contents of peanut seedlings are more sensitive to ultrasonication than polysaccharides and total carbohydrate contents. The reduction decrease in direct reducing sugars, sucrose and total carbohydrate contents were proportional with increasing time of exposure. The present data obtained showed that amylase activity of peanut seedlings and cultured cells were increased with ultrasonication, while invertase activity decreased.}, keywords = {}, url = {https://blj.journals.ekb.eg/article_64448.html}, eprint = {https://blj.journals.ekb.eg/article_64448_c33c43642d459d28bc9e62618e512438.pdf} } @article { author = {H.S, Hegazy, and S.M., Ghazi, and H.E., Daif,}, title = {STUDIES ON THE EFFECT OF ULTRASONIC WAVES ON: LIPIDS STRUCTURE AND ACTIVITY OF LIPASE IN PEANUT SEEDLINGS AND CELL CULTURES}, journal = {Biochemistry Letters}, volume = {4}, number = {1}, pages = {59-70}, year = {2008}, publisher = {Zagazig University; Faculty of Science. Center of Scientific Studies and Researches}, issn = {1687-4773}, eissn = {2974-4725}, doi = {10.21608/blj.2008.64450}, abstract = {In the present work, the effect of ultrasonic waves on lipid metabolism and lipase activity in seedlings and cell culture of peanut was carried out. The neutral lipid contents of peanut seedlings were slightly increased by ultrasonication, but the total lipid as well as phospholipids content decreased. The reduction of phospholipids and total lipid contents of peanut seedlings was accelerated by ultrasonication exposure time from 15 to 60 minutes. Lipase activity of peanut seedlings increased also with time of exposure to ultrasonic waves. Cultured cells of peanut total lipids, diacylglycerols (DG), sterols (S) and free fatty acids (neutral lipid fractions), phosphatidylinositol (PI) as well as lipase activity were increased ultrasonication, while triacylglycerols (TG) and sterol esters (SE) as well as phosphatidylethanolamine (PE), phosphatidylcholine (PC) and phosphatic acid (PA) were decreased.}, keywords = {}, url = {https://blj.journals.ekb.eg/article_64450.html}, eprint = {https://blj.journals.ekb.eg/article_64450_ddf288cfac0fd7ef2b85ee77419fdea7.pdf} }