ORIGINAL_ARTICLE
The transcriptional activity of the simian virus 40 enhancer and promoter in GH3 cells
A series of experiments was designed to investigate the activity of the SV40 early promoter and enhancer in rat pituitary tumor cells in culture but still yet not clear so, a series of enhancer promoter-reporter plasmids were constructed to study the SV40 activity in the rat pituitary tumor cell lines (GH3 and GC) then sub-cloned into pSV2(enhancer-minus)-CAT, then purified in gel and ligated into pRSV(enhancer-minus)-CAT (Sph1 restricted) then transformed into E.Coli then make restriction analysis. From the results we observed that SV40 early promoter can be activated by heterogeneous enhancers in pituitary tumor cells.
https://blj.journals.ekb.eg/article_63403_640ec309f10ff3d6ae7bd47d126965e4.pdf
2013-12-01
1
5
10.21608/blj.2013.63403
Salem
El-jaffry
1
Department of Biochemistry, Faculty of Medicine, Tripoli, Libya
AUTHOR
I.
Fehalbum
2
Department of Biochemistry, Faculty of Medicine, Tripoli, Libya
AUTHOR
Haytham.
A. A
3
Department of Biochemistry, Faculty of veterinary medicine, Zagazig University, Egypt.
AUTHOR
Mohamed M.A.
Hussein
4
Department of Biochemistry, Faculty of veterinary medicine, Zagazig University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Transcriptional activity of 3' deletion of the rat prolactin gene promoter
Regulation of gene expression of prolactin gene still a point of study for many Scientifics so this work was designed to investigate the transcriptional activity of 3- deletion of the rat prolactin gene promoter. A plasmid containing the rPrl gene promoter (-423/+38) was restricted at the unique EcoR1 site of 3' deletion of the ePrl promoter sequences in rPrl (-423/+38). With suitable condition established prPrl (-423/+38) was EcoR1 restricted in preparative amounts and Bal31 treated for optimized time periods and the resulting DNA fragments pooled. The selected positives were then restricted with BamH1, Hind III and the presence of a Bam H1/ Hind III fragment 423 bp verified by agarose gel electrophoresis. The results shows that the most proximal high affinity Pit-1/GHF-1 binding element is not an absolute requirement for GC cell specific transcriptional activity and that the Pit-1/GHF-1 binding elements with sequence to -423, is sufficient to confer relatively high transcriptional drive, in orientation independent manner
https://blj.journals.ekb.eg/article_63861_645a2c4d3c3593b8b1ba63b798ca118a.pdf
2013-12-01
6
11
10.21608/blj.2013.63861
prolactin
promoter
3' deletion
Pit-1/GHF-1
Salem
El-jaffry
1
Department of Biochemistry, Faculty of Medicine, Tripoli, Libya
AUTHOR
I.
Fehalbum
2
Department of Biochemistry, Faculty of Medicine, Tripoli, Libya
AUTHOR
Kh.
Aldawy
3
Department of Biochemistry, Faculty of veterinary medicine, Zagazig University, Egypt
AUTHOR
Mohamed M.A.
Hussein
4
Department of Biochemistry, Faculty of veterinary medicine, Zagazig University, Egypt
AUTHOR
Haytham.
A. A
5
Department of Biochemistry, Faculty of veterinary medicine, Zagazig University, Egypt
AUTHOR
ORIGINAL_ARTICLE
Effects of Zamzam water and mineral salts of treated Tap water on some biochemical parameters in male albino rats
Background: Kidney stones are one of the most painful urologic disorders. Scientists have found evidence of kidney stones in a 7,000-year-old Egyptian mummy as they may contain various combinations of elements. Zamzam water showed highly significant readings in some inorganic elements, including Na, Ca, Mg, K, HCO3, Cl, Fl, NO3, and SO4. The levels of these elements in Zamzam water may play a critical role in its effectives in the inhibition of calcium oxalate formation. Aim: This study was carried to investigate the effect of Zamzam water, and treated tab water with Ca, Mg, F, Cl and Na in adult male albino rats suffer from kidney stones induced by ethylene glycol. Materials & Methods: Kidney Calcium oxalate stones were induced in animals under study by oral administration of 0.75% of ethylene glycol dissolved in drinking water. Fifty rats were divided into equal 5 groups; each group was containing 10 rats: negative control, positive control (drinking tab water contains 0.75% ethylene glycol); negative Zamzam group (drinking Zamzam water only); Zamzam treated group (drinking Zamzam water contains 0.75% of ethylene glycol); and the last group tab water treated group (drinking treated water contain 0.75% ethylene glycol). After 4 weeks of treatment urine analysis and some biochemical analysis, were carried out in all studied groups. Results: we found that; significant differences in urine, Kidney functions and liver functions analysis in positive control group compared to negative control, while Zamzam group showed non-significance. Whereas Zamzam treated, showed a significant difference in all parameters compared to positive control group, more than tap water treated group. Conclusion: Zamzam water and treated tap water may play a critical role in its effectives in the inhibition of calcium oxalate formation.
https://blj.journals.ekb.eg/article_63862_63b7edc5ec0df33e8906d14535dcab21.pdf
2013-12-01
12
22
10.21608/blj.2013.63862
Zamzam water
kidney stones
ethylene glycol
calcium oxalate
Abdel-Khalek
L.G
1
Department of Biological Application, Nuclear Research Centre, Atomic Energy Authority, Egypt.
AUTHOR
Taha,
M.S.
2
Department of Biological Application, Nuclear Research Centre, Atomic Energy Authority, Egypt.
AUTHOR
Hassanin
W.F.
3
Department of Biological Application, Nuclear Research Centre, Atomic Energy Authority, Egypt.
AUTHOR
Shaimaa.M.
Eldeghdye
4
Department of Biological Application, Nuclear Research Centre, Atomic Energy Authority, Egypt.
AUTHOR
Akaber T.
Keshta
5
Chemistry Department, Faculty of Science, Zagazig University, Egypt.
AUTHOR
H.Y.
Mostafa
6
Chemistry Department, Faculty of Science, Zagazig University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Studies on Nanoengineered Multilayer Capsules for Drug Delivery
Background: Polymeric multilayer capsules are now being engineered to encapsulate various classes of drug molecules, by using polymers that are biodegradable or that can respond and release their payload in response to well-defined stimuli and are produced by stepwise adsorption of oppositely charged polymers onto the surface of colloidal particles followed by core dissolution, which is called Layer by Layer technique, Aim: The present study focuses on synthesis and characterization of polyelectrolyte multilayer microcapsules which was loaded with Bovine Serum Albumin and the cytotoxic effect of the microcapsules on the living cells was studied, Materials and methods: The microcapsules were fabricated by using Poly(allylamine) Hydrochloride (PAH) and Poly(styrenesulfonate) (PSS) as the counter charge polyelectrolytes and biocompatible calcium carbonate as the template which is highly promising to produce capsules for biological applications and loaded with bovine serum albumin (BSA) as a model drug, BSA labeled with fluorescein isothiocyanate (BSA-FITC), Calcium carbonate particles and the microcapsuls were characterized by SEM and TEM ,The effect of microcapsules on Madin Darby Bovine Kidney (MDBK) cell line were studied by using The 3-[4,5-dimethylthiazol-2-y]-2, 5-diphenyltetrazolium bromide MTT assay, Results: CaCO3 microparticles are very convenient template for the preparation of polyelectrolyte microcapsules 1–4μm, preloading method is more effective than post loading method , Our results confirmed that this synthetic CaCO3 microcapsules are cytocompatible, Conclusion: We thus have shown that the very easily prepared, stable, prototypical microcapsules composed of PSS/PAH are useful drug delivery agents for proof-of-principle drug delivery studies.
https://blj.journals.ekb.eg/article_63863_c94ef6f8b5153d5f7c9257e59c129de4.pdf
2013-12-01
23
37
10.21608/blj.2013.63863
Faten Z.
Mohamed
1
Biochemistry Department, Faculty of science, Zagazig university, Egypt
AUTHOR
Mohamed A.
Shemis
2
Biochemistry and Molecular Biology Dep, Theodor Bilharz Research Institute, Giza, Egypt.
AUTHOR
Mona N.
Mohareb
3
Biochemistry and Molecular Biology Dep, Theodor Bilharz Research Institute, Giza, Egypt.
AUTHOR
Heba A.
Hosiny
4
Biochemistry Department, Faculty of science, Zagazig university, Egypt
AUTHOR
ORIGINAL_ARTICLE
Evaluation of bone marrow derived mesenchymal stem cell potency on wound healing
Background: Wound healing is a complicated process of skin repair after injury. This process requires a coordinated interplay among cells, growth factors, and extracellular matrix proteins. Central to this process is the endogenous mesenchymal stem cell (MSC), which coordinates the repair response by recruiting other host cells and secreting growth factors and matrix proteins. MSCs are self-renewing multipotent stem cells that can differentiate into various lineages of mesenchymal origin such as bone, cartilage, tendon, and fat. In addition to multilineage differentiation capacity, MSCs regulate immune response and inflammation and possess powerful tissue protective and reparative mechanisms, making these cells attractive for treatment of different diseases. Aim: The beneficial effect of exogenous bone marrow-derived mesenchymal stem cell (BM-MSCs) on wound healing was observed but the mechanisms involved are barely understood. In this study, we examined signaling factors released by BM-MSCs and their effects on the cells participating in wound healing. Materials &Methods: Thirty female mice were randomly divided into three groups and these female mice were anesthetized and 6-mm full thickness, 4-cm wide deep wounds were made , then the formed colonies of the 2nd generation of BM-MSCs were injected intraperitoneally in each mice in the second group while intraregionally in the third group. These mice observed till complete healing then reverse transcription polymerase chain reaction (RT-PCR) was done for ICAM-1,ITG-β1,ITG-β2. Results: This study found that MB-MSCs promote the wound healing process through secretion of wound healing- related proteins and a broad spectrum of cytokines and growth factors, particularly vascular endothelial growth factor (VEGF).
https://blj.journals.ekb.eg/article_63864_d9e88658862acf9981cb19f335f5a4ff.pdf
2013-12-01
38
52
10.21608/blj.2013.63864
Faten
.Z.M
1
Biochemistry department, faculty of science, zagazig university, Egypt.
AUTHOR
Shimaa
H.A.
2
Biochemistry department, faculty of science, zagazig university, Egypt.
AUTHOR
Somia .
H.A
3
Biochemistry and molecular biology department, faculty of medicine, zagazig university, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Biochemical studies on olive leaves and oil against high sucrose diet in rabbits
Background: Olive leaves and oil's beneficial effects are not only related to its high content of oleic acid, but also to the antioxidant potential of polyphenolics. Objectives: The present study was aimed to assess the effects of olive leaves and oil treatments on high sucrose induced oxidative damage in rabbits. Methods: Forty male rabbits were allocated into four groups; control group was kept on normal basal ration, sucrose fed group was received 30% sucrose containing ration, sucrose plus olive leaves group was administered olive leaves 5% in ration beside sucrose 30% and sucrose plus olive oil group was received olive oil 5% in basal ration together with sucrose 30% for 60 days. Results: Feeding high sucrose diet significantly increased total lipid, triacylglycerol, cholesterol and LDL-c levels while decreased HDL-c level with hyperglycemia. High sucrose induced hepatic damage assessed by increasing liver marker enzymes. Increased testosterone, copper levels with decreased serum zinc level were also observed. Furthermore, high sucrose diet caused an induction in oxidative stress biomarkers with subsequent depletion in antioxidant enzymes (glutathione-S-transferase and catalase) and reduced glutathione level in liver, heart and kidneys. On the other hand, treatment with olive leaves or oil improved serum lipid profile, liver functions, glucose, zinc and copper levels but decreased testosterone level. Moreover, olive leaves or oil attenuated lipid peroxidation with increase in the antioxidant enzymes. Conclusion: We concluded that olive leaves as well as oil had improved the biochemical alterations induced by feeding high sucrose diet for long time.
https://blj.journals.ekb.eg/article_63865_9a3289c542351011185049a6a8750cd8.pdf
2013-12-01
53
66
10.21608/blj.2013.63865
olive oil
sucrose
Lipid peroxidation
Antioxidant enzymes
testosterone
Mohamed
Lebda
1
Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Edfina, Egypt
AUTHOR
Nabil
Taha
2
Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Edfina, Egypt
AUTHOR
Mahdy
Korshom
3
Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Edfina, Egypt
AUTHOR
Abd El-Wahab
Mandour
4
Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Edfina, Egypt
AUTHOR
Mohamed
El-Tabbakh
5
Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Edfina, Egypt
AUTHOR
ORIGINAL_ARTICLE
MODULATION OF INTERLEUKIN-6 AND IMMUNOGLOBULINS IgG AND IgM LEVELS AFTER MULTIPLE ORAL EXPOSURE TO LAMBDA-CYHALOTHRIN IN RATS
Lambda-Cyhalothrin (LCT) is a synthetic pyrethroid insecticide used worldwide in agriculture , home best , and disease vectors control . The objective of this study was to investigate the propensity of LCT to induce changes in some humoral immune parameters in adult male Sprague Dawley rats . Animals were divided into four groups (5 rats each) . The 1st and 3rd groups were orally given 1/10LD50 (12.76 mg/kg body wt.) every other day for 8 weeks. The 2nd and 4th groups were orally given dist. water every other day for 8 weeks and served as controls for the 1st and 3rd groups respectively . Blood samples were collected from each animal under ether anesthesia through heart puncture and sera were separated , at the 1st and the 15th days post LCT administration form groups 1 and 2 and groups 3 and 4 respectively . Data obtained revealed that oral LCT induced a significant decrease in IL-6, and significant increases in serum IgG and IgM concentrations. Significant increases in serum AST activity , total protein and albumin concentrations and non-significant decrease in serum ALT activity . Recovery from these changes were dependent on the measured parameter.
https://blj.journals.ekb.eg/article_63866_a2fab0bb5932a7f6baefa74b4cd3e0c2.pdf
2013-12-01
67
77
10.21608/blj.2013.63866
Nagi A.
Ibrahim
1
Zoology Department , Faculty of Sciences , Zagazig University
AUTHOR
Ahmed M.
GabAllah
2
Department of Clinical Pathology , Faculty of Medicine , , Zagazig University
AUTHOR
Alsyed R.
Farahat
3
Zoology Department , Faculty of Sciences , Zagazig University
AUTHOR