Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Association between Vitamin D Receptor Gene Polymorphisms and Anemic Patients1104759110.21608/blj.2017.47591ENFathyA. YassinChemistry Department, Faculty of science, Zagazig University, Zagazig, Egypt.Noha MohamedSaidBiochemistry Division, Chemistry Department, Faculty of science, Zagazig University, Zagazig, EgyptDohaTarekBiochemistry Division, Chemistry Department, Faculty of science, Zagazig University, Zagazig, EgyptJournal Article20170103
<span class="s1"><span class="Apple-converted-space"> </span></span><strong>Background: </strong>Vitamin D endocrine system controls calcium homeostasis and bone metabolism in addition to cellular proliferation and differentiation. Several new studies have publicized that calcitriol which represent the active form of vitamin D is involved in hematopoiesis. Furthermore, vitamin D receptor (VDR) mediates vitamin D activity. Therefore VDR gene has been proposed as one of the candidate genes for anemia. <strong>Aim: </strong>Study relationship between low hemoglobin level as the first cause for anemia and (ApaI and TaqI) polymorphisms of VDR gene in terms of genotype and allele in unrelated normal healthy individuals without chronic kidney diseases of Egyptian population. <strong>Subjects and methods: </strong>A case-control study including 130 unrelated Egyptian donors (81 cases and 49 controls) deprived of chronic kidney diseases was designed to check the relationship between VDR gene polymorphisms and low hemoglobin level. Two SNPs [ApaI (rs7975232) and TaqI (rs731236)] were typed by polymerase chain reaction (PCR) method. <strong>Results: </strong>Analyses using codominant, dominant, and recessive models failed to reveal any significant association between both (ApaI and TaqI) polymorphisms and anemia in terms of genotype and allele for the covariates (age, blood hemoglobin level, age at menopause (For women), educational level, ethnicity, medical history, serum ferritin and iron). <strong>Conclusion: </strong>VDR gene polymorphisms have no effect on anemia in Egyptian population without chronic kidney disease. Further study on the association between polymorphisms of VDR gene and anemia, a potential study design, use of large number of samples, and additional markers would improve the validity and reliability of findings.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47591_ecedc7b2de0f4be7a189af021edbdfb1.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Anticardioliptin Antibodies as a marker of hepatitis C Virus Severity11184759210.21608/blj.2017.47592ENMahmoudS. A. MetwalyChemistry Department Faculty of Science Suez Canal University,Mohy EldinA. Abdel AttyChemistry Department Faculty of Science Suez Canal University,AmalM. I. EmamChemistry Department Faculty of Science Suez Canal University,El-SaeidM. E. El-BawabBiochemistry Department Faculty of Medicine Al-Azhar University (Assuit).Journal Article20170109
<span class="s1"><span class="Apple-converted-space"> </span></span><strong><em>Background </em></strong>Anticardiolipin antibody (aCL Ab) is considered one of the contributory factors in the development of acute ischemic stroke. Chronic hepatitis C virus infection (HCV) and the antiphospholipid syndrome are two conditions that have increased the risk of stroke. The aim of this study is investigate the prevalence of anticardiolipin autoantibodies IgM (ACA IgM), in serum samples of patients with chronic HCV infection and their relationship to the severity of the viral infection. The study was performed on 75 Egyptian subjects, 25 healthy volunteers and 50 patients of both sexes; all patients were HCV-4a. And divided into two groups according to their real time (RT) PCR into, 25 patients with low viremia, (HCV-RNA) less than 10<span class="s2">6 </span>lU/ml and 25 patients with high viremia (HCV-RNA) more than 10<span class="s2">6 </span>lU/ml. All subjects after fasting for twelve hours and stored for the determination of aminotransferases (ALT, AST) and γ-GT enzymes activities, and anticardiolipin autoantibodies IgM. There is very highly significant increase of serum ACA (IgM) MPL (U/ml) in patients with high viremia and low viremia when compared to the control group (p <0.0001 for both) and high significant increase in patients with high viremia when compared with low viremia (p <0.0001). Also there is high significant correlation between serum ACA (IgM) and quantity of HCV-RNA in both high and low viremia patients. But no significant correlation between serum ACA (IgM) serum ACA (IgM) and serum liver enzymes.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47592_1d5cec0f6bf57c32afbd5c70d83c2982.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Correlation Between Hepatitis C and Hyperglycemia In Patients on Regular Hemodialysis19274759310.21608/blj.2017.47593ENMostafaA. M. AshourMsc . Biochemistry, chemistry department, faculty of science (Ismailia), Suez Canal University, Egypt.MohyEldinA. Abdel AttyProfessor of organic chemistry, Chemistry department, Faculty of Science, Suez Canal University, Egypt.El-SaeidM. E. El-BawabProfessor of Biochemistry and head of Biochemistry Department, Faculty of Medicine Al-Azhar University (Assuit), Egypt.AmalM. I. EmamLectrature of organic chemistry, Chemistry department, Faculty of Science(Ismailia), Suez Canal University, EgyptJournal Article20170103
<span class="s1"> </span><strong>Background: </strong>Hepatitis C virus (HCV) –is the leading cause of chronic liver disease worldwide. Many studies have shown an association between hepatitis C and type 2 diabetes mellitus (DM2) and increase of insulin resistance could play a crucial role. Glucose homeostasis may become extremely difficult because most oral hypoglycemic drugs are contraindicated if hepatic or renal function were reduced. Thus, insulin remains the elective treatment. Insulin-resistance (IR), HCV infection, carbohydrate metabolism disorders and difficulties in dietary management, make to be achieved specially in those with renal function impairment or failure. <strong>Aim: </strong>The present study aims to investigate the correlation between the hepatitis C affection (in chronic renal failure patients on regular hemodialysis ) and Hyperglycemia as a case control study in Egyptian patients.. <strong>Methods: </strong>The study group consisted of 70 patients who were on regular hemodialysis therapy (group (1): a 35 HCV positive and group (2): a 35 HCV negative). Each group was subdivided according to HOMA' score into HOMA(+) with HOMA score >2.5 and HOMA(-)with HOMA score < 2.5. All patients and normal control group (n =35) were subjected to the estimation of fasting blood sugar(glucose) (FBS), alanine transaminase (ALT), aspartate transaminase (AST), and fasting serum insulin, HOMA score <strong>Results: </strong>serum insulin levels, ALT, AST, and duration of hemodialysis of HCV +ve patients were statistically significantly higher than those of HCV -ve. and activities of ALT, AST, and HOMA score in HCV-ve patients were significantly higher than those in normal controls. Among HOMA score (+) patients there was a statistically significant increase of serum insulin and HOMA score in HCV +ve group than HCV -ve group. Among HCV +ve patients there were statistically significant correlation between ALT and both insulin level and HOMA score. Conclusion: Hepatitis C virus infection enhances insulin resistance in chronic hemodialysis Egyptian patient.<span class="Apple-converted-space"> </span>
https://blj.journals.ekb.eg/article_47593_a3fbc2c1a37a7f57b29db31a14550424.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Protective Role of Qurecitin against Zinc Oxide Nanoparticles Induced Hepatotoxicity30394759410.21608/blj.2017.47594ENMonaM. LotfyMaster student faculty of Vet. Med. Suez Canal University, Ismailia, EgyptIbrahimA. IbrahimDepartment of Biochemistry, Faculty of vet. Medicine, Suez Canal University, Ismailia, EgyptSherifY. SalehDepartment of Biochemistry, Faculty of vet. Medicine, Suez Canal University, Ismailia, EgyptMarwa A.ElbeltagyDepartment of Biochemistry, Faculty of vet. Medicine, Suez Canal University, Ismailia, EgyptHaytham A.AliDepartment of Biochemistry, Faculty of vet. Medicine, Zagazig University, Zagazig, EgyptJournal Article20170202
<span class="s1"><span class="Apple-converted-space"> </span></span><strong>Background: </strong>The extensive use of nanoparticles in our life especially zinc oxide nanoparticles (ZnO NPs) in cosmetics, sun blockers, paints and food additives draw our attention toward their hepatotoxicity and the protective role of Qurecitin (QE).<span class="Apple-converted-space"> </span>
<strong>Objective: </strong>the present study aimed to investigate the hepatotoxic effect of ZnONPs and the protective role of Qurecitin against it.<span class="Apple-converted-space"> </span>
<strong>Results: </strong>I/P injection of ZnONPs in a dose of 200 and 400 mg/ kg b.wt resulted in a significant increase in the serum levels of ALT, ALT and ALP with a significant increase in the hepatic MDA levels and decrease in non-enzymatic (GSH) and Enzymatic (GPx, GR, SOD and CAT) activities and their mRNA levels. Meanwhile, the co-treatment of ZnONPs with QE ameliorate the hepatic condition through decreasing the liver enzymes and MDA with increase in the antioxidant levels and their gene expression.<span class="Apple-converted-space"> </span>
<strong>Conclusion: </strong>Taken together it can be concluded that QE has an ameliorative effect against ZnONPs hepatotoxicity.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47594_a76ce209f362d4d61d82cb6409347fe8.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Assessment of Transforming Growth Factor-β1 in Egyptian Patients with Chronic Hepatitis-C Virus and Hepatocellular Carcinoma40484759510.21608/blj.2017.47595ENFatenZahranChemistry Department, Faculty of Science, Zagazig University, EgyptHodaEl-EmshatyGastroenterology Center, Mansoura University, EgyptMonaGouidaMansoura Children Hospital, Mansoura University, EgyptMohamedHussienMansoura Chest Hospital, EgyptJournal Article20170209
<span class="s1"><span class="Apple-converted-space"> </span></span><strong>Background: </strong>Hepatocellular carcinoma (HCC) is the end-stage of chronic liver diseases (CLDs). The main aetiologies of CLDs are chronic hepatitis C virus (HCV) infection. <strong>Objective: </strong>The aim of this work was to evaluate diagnostic value of TGF-β1 in patients with liver Fibrosis and HCC. <strong>Materials and Methods<em>: </em></strong>In the present study, we have investigated 51 patients with Fibrosis (F2-F4), 30 (HCC) patients, in addition 40 normal healthy individuals were enrolled in this study as control group. Laboratory liver fibrosis indices including FIB-4 ratio, APRI ratio, and AST/ALT ratio were calculated in all studied groups. AFP and carcinoembryonic antigen (CEA) were estimated in all studied groups. TGF-β1 was evaluated using Flow Cytometry technique. <strong>Results: </strong>There was high significance difference in APRI, FIB-4 between HCC, Fibrosis patients comparing with Control group (p<0.05), also there was high prevalence TGF-β1 in HCC patients comparing with both Fibrosis and healthy control group (P<0.005). <strong>Conclusion: </strong>TGF-β1 has diagnostic value in assessment of hepatocellular carcinoma.<span class="Apple-converted-space"> </span>Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201The effect of Saffron aqueous extract on hepatocellular carcinoma rat model49634759610.21608/blj.2017.47596ENNabilaZeinBiochemistry Division, Chemistry Department Faculty of Science, Zagazig University, EgyptJournal Article20170208
<span class="s1"><span class="Apple-converted-space"> </span></span>The aim of this study was to investigate the tumor suppressive effects of saffron in an experimental hepatocellular carcinoma (HCC) model in rats. Thirty rats were included in this study and divided into:- Control group : Rats which receive distilled water only. HCC group : induced by diethylnitrosamine (DEN) and CCl<span class="s2">4 </span>only. Saffron group : Rats were administered orally saffron respectively 2 weeks prior to HCC initiation and continued for 6 weeks. The effect of saffron on ALT and AST activity and albumin level were studied also TGF-beta level and the TAC level were estimated and induction of apoptosis was determined by histopathological studies. Treatment with saffron was significantly decreased the activities of ALT and AST also increased the albumin level, decreased the TGF-beta level and increased the TAC level, it also reduced the elevation in the number and the incidence of hepatic dyschromatic nodules. Moreover, treatment with saffron extract brought significant restoration of the hematological parameters to values that were comparable to those of the negative control group, it also enhances the liver and kidney health, this study provides evidence that saffron exerts anti-oxidant, chemoperventive effect against liver cancer through induction of apoptosis as appeared in histopathological studies<span class="Apple-converted-space"> </span>Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Biosynthesis and Characterization of Lead Sulfide Nanoparticles Using Wastewater Bacteria64844759710.21608/blj.2017.47597ENOmar A.RamadanBiochemistry Division, Chemistry department, Faculty of Science, Zagazig University, EgyptAshraf A.SabryAssistant Professor of microbiology, Botany department, Faculty of Science, Zagazig University, Egypt.A. T.KeshtAssistant Professor of biochemistry, Chemistry department, Faculty of Science, Zagazig University, Egypt.A. A.AmerProfessor of physical ochemistry, Chemistry department, Faculty of Science, Zagazig University, Egypt.Journal Article20170212
<span class="s1"><span class="Apple-converted-space"> </span></span><strong><em>Background: </em></strong>Wastewater considered as a lost economical value, due to it contains a huge content of bacteria that could biosynthesis of a valuable materials from hazardous material. Heavy metals that found in wastewater considered as row material for bacteria to biosynthesis nanoparticles as lead sulfide (PbS) which used as semiconductor<strong><em>. Aim: </em></strong>The present study aims to biosynthesis and characterization of lead sulfide nanoparticles using bacteria isolated from wastewater <strong><em>Materials & Methods: </em></strong>Sampling different samples from three places at different stages in the wastewater plant. Samples were collected from influent (raw sewage), from the outlet of the 2ry sedimentation tank (after biological treatment) and from the outlet after chlorination (effluent). Determine of physico-chemical characteristics of the wastewater samples. Isolate some of bacteria present in these different stages of treatment in the plant. Study the capabilities of the isolated strains for lead resistance. Determine the minimum inhibitory concentration of selected bacterial strains that are resist to lead concentration. Biosynthesize lead nanoparticles using the selected bacterial isolate. Characterize the lead nanoparticle produced by selected bacterial isolate. <strong><em>Results: </em></strong>The biosynthesis of lead nanoparticles by <em>Serratia plymuthica, </em>which isolated from Zenine wastewater treatment plant, Giza, Egypt. <em>Serratia plymuthica </em>had ability to resist lead till 80mg/l and had ability to biosynthesize lead nanoparticle. UV-Vis spectroscopy results for pellets of <em>Serratia plymuthica </em>inoculated in Tryptic soy bean broth (TSB) containing 80 mg/l of Pb(NO<span class="s2">3</span>)<span class="s2">2</span>show formation of peak at ~ 330 nm, which was a specific peak for lead nanoparticles. TEM image for pellets of <em>Serratia plymuthica </em>inoculated in TSB containing 80 mg/l of Pb(NO<span class="s2">3</span>)<span class="s2">2</span>show formation of Pb NPs intracellular and extracellular and cells aggregation. Dynamic light scattering (DLS) results show ability of <em>Serratia plymuthica </em>to biosynthesis PbNPs with mean diameter 92.93 nm. X- ray diffraction (XRD) results show ability of <em>Serratia plymuthica </em>to biosynthesis PbS which had semiconducting properties and used in solar cells manufacturing. <strong>Conclusions: </strong>The results approved the biosynthesis of PbS nanoparticles by <em>Serratia plymuthica </em>which isolated from wastewater.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47597_fe2c2247d8ed1afb87630f2fcb6cc900.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Shistosoma Mansoni-infected mice: The progression of nitric oxide and superoxide anion production in activated peritoneal macrophages.85944759810.21608/blj.2017.47598ENMayssaa M.WahbyDepartment of Biochemistry, Faculty of Science, Alexandria UniversityKamal M.KandeelDepartment of Biochemistry, Faculty of Science, Alexandria UniversityEmanRashwanDepartment of Immunology, Medical Research Institute, Alexandria UniversityEnas R.MostafaJournal Article20170301
<span class="s1"><span class="Apple-converted-space"> </span></span><strong><em>Background: </em></strong>The cell mediated immunity has revealed the importance of activated macrophages as key immune effector cells. Activated macrophages have the ability to generate reactive oxygen and nitrogen species (ROS and RNS) to kill parasite. <strong><em>Objectives: </em></strong>The current study aimed to investigate the progression of nitric oxide (NO) and superoxide anion (O<span class="s2">2-</span>) production in activated macrophages, isolated from Schistosoma Mansoni<em>-</em>infected mice during innate immune response. <strong><em>Methods: </em></strong>Hundred male albino mice were divided into two main groups; control and infected. The mice of the infected group were injected subcutaneously via the tail with one hundred cercariae /mouse. <em>In vivo </em>production of NO and the O<span class="s2">2- </span>were estimated in the isolated peritoneal macrophages with the progression of post infection time. <strong><em>Results: </em></strong>The number of the isolated peritoneal macrophages and the production of NO and O<span class="s2">2- </span>were significantly increased exponentially with time intervals throughout eight weeks of infection compared to the control. The highest levels of both the peritoneal macrophages and NO as well as the lowest O<span class="s2">2- </span>value were shown at the 8<span class="s2">th </span>week post infection. The correlation analysis showed significant positive relationship between the number of peritoneal macrophages and NO production and non-significant positive correlation between their numbers and O<span class="s2">2- </span>production. In contrast, the NO and O<span class="s2">2- </span>production showed significant negative correlation in the activated cells. <strong><em>Conclusion: </em></strong>The activated macrophages are important immune effector cells that are capable of generating cytotoxic molecules such as NO and O<span class="s2">2- </span>during the prepatent period of Schistosoma Mansoni infection. However, the NO plays the key role in this innate immune response..<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47598_5d7f842baf4707ee20b1da7a6b06342b.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Production, Purification and Characterization of Polygalacturonase from Bacillus licheniformis SHG10951094759910.21608/blj.2017.47599ENNadia Z.ShabanDepartment of Biochemistry, Faculty of Science, Alexandria UniversityTayssir M.GhonaimDepartment of Biochemistry, Faculty of Science, Alexandria UniversityAliaa A.MasoudDepartment of Biochemistry, Faculty of Science, Alexandria UniversityNabilEltokhyCity of Scientific Researches and Technological Applications, Borg El Arab, Alexandria, EgyptAmira M.EmbabyDepartment of Biotechnology, Institute of Graduate studies and Research, Alexandria UniversityJournal Article20170309
<span class="s1"><span class="Apple-converted-space"> </span></span><strong><em>Background</em>: </strong>Microorganisms are the best source of pectinolytic enzymes as they allow an economical technology with low resource consumption. <strong><em>Objectives: </em></strong>The present study was carried out to isolate, purify and characterize polygalacturonase (PGase) form <em>Bacillus licheniformis </em>SHG10. <strong><em>Methods: </em></strong>The concentrated dialysed cell free extract was loaded on prepacked DEAE-Sepharose Fast-Flow ion exchange chromatography. The active PGase fractions were concentrated and loaded again on sephacryl Fast-Flow High Resolution (FF S-100 HR) chromatography. Molecular weight was determined using slab-gel SDS-Polyacrylamid gel electrophoresis and Characterization of the purified enzyme was performed. <strong><em>Results: </em></strong>The results showed that the enzyme was purified with a purification fold (33.34) and yield (41.73%) with specific activity (8.67 μ moles/min/mg protein). It has a molecular mass of about 68.0 kDa. While, on SDS-PAGE electrophoresis, the purified PGase appeared as single band with molecular mass of about 34.0 kDa, suggesting that the purified PGase is a dimer protein. The purified enzyme exhibited maximal activity at a temperature of 45<span class="s2">o</span>C and pH 8.5. The maximum velocity of the enzyme in presence of citrus pectin and pectate as substrates were 10.98 and 14.7 μ mol galacturonate/min/mg protein, respectively. The Michaelis constant (Km) values were 0.085 and 0.039 mM, respectively, indicating that the purified PGase has higher affinity to pectate (non-methylated pectic substance) than citrus pectin as substrate.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47599_9cfc73f352e1cc4272c5ff72a4aec6f7.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201The Effect of Multidrug System on Treatment of Hepatocellular Carcinoma1101234760010.21608/blj.2017.47600ENAhmad R.BassiounyBiochemistry Departement, Faculty of Science, Alexandria University, Alexandria, EgyptAmira Z.HassanBiochemistry Departement, Faculty of Science, Alexandria University, Alexandria, EgyptTayssir M.GhonaimBiochemistry Departement, Faculty of Science, Alexandria University, Alexandria, EgyptFatma A.MahmoudBiochemistry Departement, Faculty of Science, Alexandria University, Alexandria, EgyptJournal Article20170311
<span class="s1"><span class="Apple-converted-space"> </span></span><strong>Background: </strong>Hepatocellular carcinoma is the most frequent primary malignancy of the liver. It is the sixth most common cancer and third most frequent cause of cancer-related death worldwide. <strong>Objective: </strong>Epigenetic mechanisms (such as methylation, acetylation, and ubiquitination) are altered in HCC. One of these epigenetic mechanisms is Histone acetylation. The balance between histone transacetylases and deacetylases is often damaged in cancer, leading to changed expressions of tumor suppressor genes and/ or proto-oncogenes. <strong>Methods: </strong>HepG2 cells were treated with curcumin, valproic acid and curcumin + valproic acid than subjected to MTT assay, DNA fragmentation and real time PCR. <strong>Results: </strong>The result from MTT assay showed that IC<span class="s2">50 </span>of curcumin = 0.91 mM, valproic acid = 2.61 mM, curcumin + valproic acid = 1.12 mM. Real time PCR showed decrease in HDAC1, VEGF, IL- 6 and increase in PTEN, P53 genes expression in cell treated with curcumin or valproic acid individually while treatment of both show no synergism<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47600_7bd5f96dc891be94ecc19d005ac72e10.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Apoptotic effect of silver nanoparticles in rat1241354760510.21608/blj.2017.47605ENI.AshourBiochemistry Department, Faculty of Vet. Medicine , Suez Canal University, EgyptSaleh A. ,ShalbyBiochemistry Department, Faculty of Vet. Medicine , Suez Canal University, EgyptE.IbrahimAnimal Health Research Institute , Agriculture Research centre, Dokki , Egypt.MDowidarAnimal Health Research Institute , Agriculture Research centre, Dokki , Egypt.Journal Article20170504
<span class="s1"><span class="Apple-converted-space"> </span></span><strong>Background: </strong>Silver nanoparticles (AgNPs) was recommended as a disinfectant and can be used as drug in the treatment of some non- curable disease in lives stock. Aim: the toxicity of nanosilver is not well known, it is essential to examine its safety. Material and methods: fifty male rats were used to assess the dose – dependent manner effects of AgNPs. Adult male rats were divided randomly into five groups, in the four experimental groups, nanosilver particles were injected intrapretoneally for 30 consecutive days at doses of 0.25, 0.50, 1.0 and 2.0 mg/kg 1 B.W. Rats in control group received equal injections with deionized water. Liver sample were collected and stored in liquid nitrogen tank for estimation of some gene expressions. Results: AgNPs administration showed a significant increase in the gene expressions of TNF-α, P<span class="s2">53 </span>and caspase -3 levels conclusions: It was concluded that injection of AgNPs in high doses for long period produced changes in gene expression and apoptotic effects were indicated<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47605_d2ff6718807a04a94c093680b7ee46c4.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Evaluation of Tumor Necrosis Factor-α, Lactate Dehydrogenase and Gamma -Glutamyl Transferase as Independent Predictors for Monitoring Progressive HCV-Chronic Liver Disease1361464760610.21608/blj.2017.47606ENHoda MohamedEl-EmshatyGastro-Enterology Surgical Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt,Mohy Eldin AbdelfatahAbdel AttyChemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt,Talat AbdallahIbrahimGastro-Enterology Surgical Center, Faculty of Medicine, Mansoura University, Mansoura, EgyptYaser MostafaElgazarGastro-Enterology Surgical Center, Faculty of Medicine, Mansoura University, Mansoura, EgyptJournal Article20170510
<span class="s1"><span class="Apple-converted-space"> </span></span><strong><em>Objective: </em></strong>Hepatocellular carcinoma (HC) ranks as the 5<span class="s2">th </span>most common malignant cancer and the 3<span class="s2">rd </span>most frequent cause of death worldwide. The majority of HCC patients are not amenable to curative therapy as they are detected at late stages. Therefore, our aim was to evaluate the clinical implications of TNF-α level and its correlation with the activities of GGT and LDH in monitoring the progression of HCV-chronic liver disease.<span class="Apple-converted-space"> </span>
<strong><em>Materials and methods</em></strong>: This study comprised forty eight patients suffering from HCV-chronic liver disease; 24(50%) cases with HCC, 14(29.2%) cases with LC and 10(20.8%) cases with CH. Twenty five healthy individuals (HI) served as control group. Sera of all individuals were examined for the activities of TNF-α, LDH, GGT, AFP and its correlation with other laboratory investigations.<span class="Apple-converted-space"> </span>
<strong><em>Results</em></strong>: Serum levels of TNF-α, LDH, AFP were elevated significantly in HCC patients compared to LC and CH but the difference between LC and CH was elevated significantly only (p<0.0001) in TNF-α. Significant association was recorded between LDH and TNF-α, GGT, AFP, ALT and ASTlevels. Linear regression for TNF-α, LDH and AFP showed significant prediction for progression of HCV-chronic liver disease. <strong><em>Conclusion</em></strong>: Serum level of LDH and TNF-αcould be used simultaneously with AFP for the evaluation of chronic inflammation leading to cancer development.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47606_a5a013b5df18d1bc93075b9227f1026c.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Anti-tumor activity of phloretin in treatment of induced hepatocellular carcinoma in rats.1471714760710.21608/blj.2017.47607ENMohamed H.SherifChemistry Department, Faculty of Science, Zagazig University, EgyptAl-Shimaa M.AbasBiochemistry Division, Chemistry Department, Faculty of Science, Zagazig University, EgyptDalia S.AbdrabouhBiochemistry Division, Chemistry Department, Faculty of Science, Zagazig University, EgyptJournal Article20170603
<span class="s1"><span class="Apple-converted-space"> </span></span><strong>Background: </strong>Hepatocellular carcinoma (HCC) is a major health problem, classified among the higheast five most widespread malignancies. <strong>Objectives</strong>: this experimental study aims to assess the anti-tumor activity of phloretin in treatment of induced HCC in rats. <strong>Methods: </strong>fifty adult male albino rats were classified into 5 groups (n=10, each). Group <strong>I </strong>(control group), Group <strong>II </strong>(DMSO group). Group <strong>III </strong>(preventive) (pre-treated with phloretin for 14 day before HCC induction and continued during induction period) .Group <strong>IV </strong>was HCC – Induced (DENA) group. Group <strong>V </strong>(therapeutic group) (treated with phloretin after HCC induction peroid). At the end of the experiment blood serum samples and liver tissues were collected. <strong>Results: </strong>the results showed that DENA caused liver damage as proved by significant high increase in (ALT), (AST), (ALP) (GGT) activities and marked significant decrease in albumin content. Also, induced oxidative stress as pointed out an increase in (MDA) and (NO) level and decrease in (GSH), (GST) and (CAT) activity compared with the control values. Also, it decreased apoptotic pathway by decrease in Caspase-3 and Caspase-8 concentration. Treatment with phloretin significantly reduced the elevation in liver enzymes and oxidative stress; it also induced apoptosis by significant increase in Caspase-3 and Caspase-8 concentration compared to HCC group. <strong>Conclusions: </strong>this study suggests that phloretin plays an important role in protection against DENA induced HCC.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47607_a05c2d77ac8fb92041821b3969b2cca1.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Silver Nanoparticles Effect on Cytokines in Rat1721824760810.21608/blj.2017.47608ENI.AshourBiochemistry Department, Faculty of Vet. Medicine , Suez Canal University, EgyptS,SalehBiochemistry Department, Faculty of Vet. Medicine , Suez Canal University, EgyptA. ,ShalhyBiochemistry Department, Faculty of Vet. Medicine , Suez Canal University, EgyptE.IbrahimAnimal Health Research Institute , Agriculture Research centre, Dokki , EgyptM.,DoiwdarAnimal Health Research Institute , Agriculture Research centre, Dokki , EgyptJournal Article20190612
<span class="s1"><span class="Apple-converted-space"> </span></span><strong>Background: </strong>Silver nanoparticles (AgNPs) was recommended in medicine and veterinary medicine fields, and can be used as drugs for treatment of some diseases. Aim: the toxicity of nanosilver is not well known, it is essential to examine its safety. The experimental albino rats were divided into five groups , each group comprising of ten rats, after adaptation in the animal houses in Faulty of Vet. Medicine Suez Cana University for one week. The groups named as N1, 2, 3, 4 and 5. Group N1, was called control group and was gives deionized water. Blood samples were collected at the end of experiment; serum samples were separated for determination the values of IL-Lβ, IL-2, IL-6, IL-10 and TNF-α. Ag-NPs administration showed a variable significant increase in the levels of Il-Lβ, IL-2, IL-6, IL10 and TNF- α . Conclusion : Nanoparticles induce inflammatory immune responses at lower concentrations and chemokines are the major cytokines induced at early stage of exposure to silver nanoparticles..<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47608_f3647ce0e2ae95c5525c64f7b7aa08e0.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Inulin activity against Ehrlich Asites Carcinoma1831974760910.21608/blj.2017.47609ENReda M.FekryDepartment of chemistry, Branch of organic chemistry, Faculty of science, Zagazig University, EgyptAkaberT.HusseinDepartment of chemistry, Branch of Biochemistry, Faculty of science, Zagazig University, EgyptRadwa A.SaadoBSc. of Biochemistry, Faculty of science, Zagazig University, EgyptJournal Article20170711
<span class="s1"><span class="Apple-converted-space"> </span></span><strong>Background</strong>: Inulin is a natural storage carbohydrate has benefits effect largely related to its chemical structure as it has antitumor and antioxidant activity. <strong>Objectives</strong>: This study aims to investigate the antitumor & antioxidant activities of inulin against Ehrlich Ascites Carcinoma (EAC) in female mice, also study the effects of inulin on liver and kidney tissues. <strong>Materials & Methods</strong>: forty female Swiss albino mice were divided into four groups each group include ten mice: Group 1 “negative control group” mice were injected with sterile saline, group 2 “Positive control group” mice injected with EAC, group 3 “Therapeutic group” mice injected with EAC then inulin, and group 4 “Preventive group” in which mice were injected with inulin then EAC. The most effective dose of inulin was determined, viability test and life span were performed. Also, antioxidants, liver and kidney functions were measured. <strong>Results</strong>: inulin was safe compound with the most effective dose (5 mg/kg), inulin treatment showed a significant inhibitory on EAC count and volume in both preventive and therapeutic groups as well as, it recorded a significant reduction in anti-oxidants ( malondialdehyde, total antioxidant capacity, superoxide dismutase, catalase, and reduced glutathione), with slightly changes in liver & kidney tissues. <strong>Conclusion: </strong>Inulin has strong effect against EAC because of its chemical structure that responsible for its antioxidant activity.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47609_d3ae926667c561af1e9134870caf3c8b.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Cardiac Status in Hepatitis B Virus and Hepatocellular Carcinoma in Egyptian Adolescents1982084761010.21608/blj.2017.47610ENWaleed MohamedSeragChemistry Department, Faculty of Science, Suez University, Suez, EgyptEman MohamedElsayedPediatric department, Faculty of Medicine, Ain Shams University, Cairo, EgyptJournal Article20170710
<span class="s1"><span class="Apple-converted-space"> </span></span>Hepatitis B virus (HBV) is the major risk factor of hepatocellular carcinoma (HCC) in all age groups. There is a need to find the effect of them on the cardiac status in adolescence age by laboratory and physical examination for assessment of different outcomes of both diseases. Cross sectional study included 15 adolescents with HBV, another 15 with HBV complicated by HCC and 15 healthy persons served as control with age range 12 up to 18 years (14.4±3.58). They were selected for laboratory interleukin 6 (IL-6), Troponine I and Creatin Kinase MB fraction (CK MB) testing and further were examined at the Cardiology Unit in Pediatric department of Ain Shams University hospitals for complete echocardiographic and anthropometric evaluation.<span class="Apple-converted-space"> </span>
15 patients have HCC with HBV were examined with mean age 14.15±3.01 years and another 15 are having HCV (14.64±4.13 years). The duration of HCC was 2.16 ± 2.89 years while the exposure of HBV was 2.27 ±3.35 years in the HCV group. Differences were found between both groups regards IL-6 as mean values were 0.26 pg/ml in HBV and 0.721 pg/ml in HCC group while no differences between groups were detected regards cardiac markers ; troponine I and CK MB and anthropometric evaluations. In adolescent patients with HBV and HBV with HCC , there is no change in the cardiac status.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47610_5e338514e96dc25564b907731adba6bc.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Plasma and Urinary NGAL-2 as Early Biomarkers for Diagnosis of Acute Kidney Injury in Pediatric Post-Operative Cardiac Patients2092204761110.21608/blj.2017.47611ENWaleed M.SeragChemistry Department, Faculty of Science, Suez UniversityWaleed M.El-GuindyFaculty of Medicine, Ain Shams UniversitySahar S.El-SakkaChemistry Department, Faculty of Science, Suez UniversityEbtsam S.KotbChemistry Department, Faculty of Science, Suez UniversitySherookIprahimCentral Blood Bank, Ministry of Health and Population , SuezJournal Article20170909
<span class="s1"><span class="Apple-converted-space"> </span></span>Acute kidney injury (AKI) is one of the critical multiples after pediatric cardiac surgery. The diagnosis of post-operative AKI is usually established based on elevated serum creatinine. Novel, effective and early diagnostic markers of AKI are still needed to be established. The aim of this study is validate new and faster biomarkers for early diagnosis of AKI. The survey population composed of 30 children aged from week to 204 months with a mean of months = 43.08 ± 52.15. These children were subjected to cardiac surgery with cardiopulmonary bypass (CPB).Then they were divided into AKI and non-AKI subgroups according to the post-operative status. Surveillance of the patients was achieved by subsequent measurements of both plasma and urinary NGAL, serum creatinine (S.Cr) and serum urea levels at 2 hours (h) and 24 h, postoperative. Additionally, only serum creatinine and serum urea were measured at 48 hours post-CPB. No significant differences between both groups<span class="Apple-converted-space"> </span>
concerning the S.Cr levels at baseline (p=0.572,AUC=0.566), 2 h (p=0.619, AUC=0.558) and 24 h (p=0.197, AUC=0.651) while a high significant difference was observed at 48 h post-operative (p<0.001 , AUC=1.000) .No significant differences between the two groups were detected concerning the serum urea levels at baseline (p=0.455, AUC=0.587), 2 h (p=0.113 , AUC=0.685) and 24 h (p=0.129, AUC=0.677) while a high significant difference at 48 h (p<0.001 , AUC=1.000). There was no significant differences concerning the plasma and urinary NGAL levels measurements at baseline (p=0.215) while a highly statistically significant difference was spotted at 2 h (p<0.001) and at 24 h (p<0.001). Plasma and urinary NGAL serve as early predictors of AKI before the elevation of S.Cr. in postoperative patients.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47611_7409713929b008175fff4228f0734208.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201MiRNAs-181a/b as Predictive biomarkers for olaparib sensitivity in triple-negative breast cancer cells.2212294761210.21608/blj.2017.47612ENAbeer M.AshmawyDepartment of Cancer Biology, National Cancer Institute, Cairo University, Cairo 11796, Egypt.Mona A.ShetaDepartment of Cancer Biology, National Cancer Institute, Cairo University, Cairo 11796, Egypt.FatenZahranChemistry Department, Faculty of Science, Zagazig University, Egypt.Abdel Hady A.Abdel WahabDepartment of Cancer Biology, National Cancer Institute, Cairo University, Cairo 11796, Egypt.Journal Article20170910
<span class="s1"><span class="Apple-converted-space"> </span></span><strong>Background: </strong>spotting the scope on triple-negative breast cancer (TNBC) as the most aggressive type of breast cancer, with no targeted therapeutic options. TNBC is often characterized by having defects in DNA repair due to defects in BRCA making this cancer a rational target for the synthetic lethality of olaparib, as an inhibitory target agent of the alternative DNA repair pathway (poly ADP-ribose polymerase “PARP” inhibitor). <strong>Objectives</strong>: the present study aims to evaluate the value of miRNAs-181a/b as potential biomarkers in predicting the response of TNBC to olaparib. <strong>Methods: </strong>anti-miRNAs-181a/b was transfected into MDA-MB-231 cell line using HiPerFect transfection reagent, the transfected and untransfected cells were subjected to olaparib. The effect of miRNAs-181a/b on MDA-MB-231 treated cells with olaparib was evaluated through the detection of essential proteins involved in apoptosis and cell proliferation including Caspase-8, Bcl-2, and Ki-67. Further, the expression level of ataxia telangiectasia mutated (ATM) was determined as a functional target of miRNAs-181a/b. <strong>Results: </strong>a significant decrease in Caspase-8 activity, and Bcl-2, but a significant increase in cell survival, cell proliferation, and ATM protein were observed upon suppression of miRNAs-181a/b by their inhibitors followed by treatment with olaparib for TNBC cell line (MDA-MB-231 cells). <strong>Conclusions: </strong>our data confirmed that miRNA-181a and miRNA-181b play a critical role for detecting the sensitivity of TNBC cells to olaparib. As well as miRNAs-181a/b could be used as a potentially predictive biomarkers for response to olaparib.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47612_ed3affa3e4b3b821de76f1e25093ce4a.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201Protective Effect of Ginger Extract against Cisplatin-Induced Nephrotoxicity in Rats2302474761310.21608/blj.2017.47613ENMohamed H.SheriffChemistry Department, Faculty of Science, Zagazig University, EgyptAl-shimaa M.AbasBiochemistry Division, Chemistry Department, Faculty of Science, Zagazig University, EgyptBasem M.Abd- El- RahmanBiochemistry Division, Chemistry Department, Faculty of Science, Zagazig University, EgyptJournal Article20171007
<span class="s1"><span class="Apple-converted-space"> </span></span><strong>Background</strong>: Nephrotoxicity was reported in the initial clinical trials of cisplatin chemotherapy. <strong>Aim of study: </strong>Our study was designed to evaluate the nephroprotective effect of Ginger extract promoted by cisplatin. <strong>Material & Methods: Animals </strong>were divided into7 groups as follow: Group1 (Control)<strong>: </strong>Animals were injected intraperitoneal with 1ml single saline dose.Group2 (DMSO): Animals were administered orally with1 ml of 6.5% DMSO for 19 days .Group3 (cisplatin): Animals were injected intraperitoneal with cisplatin (10 mg/kg) as single dose to induce nephrotoxicity. Group 4 (Ginger only): Animals were administrated orally with 600 mg/Kg/day of ginger extract for 19 days. Group 5, 6 and7: Animals pretreated with oral dose of ginger extract (200, 400 and 600 mg /kg/day respectively) for two weeks before and 5 days after single IP cisplatin (10 mg/kg). <strong>Results: </strong>Administration cisplatin caused significant elevation in serum urea, creatinine concentration and kidney tissue levels of MDA and NO. Also caused significant increase in Caspase-3 levels, cisplatin significantly decreased level of Na. it decreased K, Mg and Ca level but statistically non-significant. Also it significantly decreased the antioxidant level of Catalase. Treatment with Ginger extract restored the levels of kidney concentration also oxidative stress markers in groups 5, 6 and 7 and also decrease Caspase-3 level. Also histopathological analysis confirmed that. <strong>Conclusion: </strong>ginger extract have a protective role in cisplatin induced nephrotoxicity.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47613_7a83bb728e772b2c9c987b193b967a7a.pdfZagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477313120171201A genetic and biochemical study of obesity in Egypt2482644761410.21608/blj.2017.47614ENNoha MohamedSaidBiochemistry Division, Chemistry Department, Faculty of science, Zagazig University, Zagazig, EgyptFathyYassinChemistry Department, Faculty of science, Zagazig University, Zagazig, EgyptSaraSelimBiochemistry Division, Chemistry Department, Faculty of science, Zagazig University, Zagazig, EgyptJournal Article20171012
<span class="s1"><span class="Apple-converted-space"> </span></span>Background: Numerous studies have documented the association adiponectin (ADIPOQ) gene polymorphisms with obesity but the consequences are indecisive. The purpose of this work is to study the connection between adiponectin gene polymorphisms G276T and T45G with obesity and biochemical variants in Egyptian population. Material and methods: Genotyping of G276T single nucleotide polymorphisms of adiponectin gene was performed by Alleles Refractory Mutation Systems PCR(ARMS PCR) method, while Genotyping of T45G single nucleotide polymorphism of adiponectin gene was performed by RFLP PCR method. The analysis was done in 172 matched person; 89 obese patients, 43 overweight patients, and 40 normal healthy controls. Biochemical parameters were calculated through standard procedures. Results: For adiponectin polymorphism G276T, it was related to overweight under Co dominant and recessive models (P= 0.010, P= 0.036) respectively, also it was associated with obesity under recessive model (P= 0.048). Higher BMI was associated with heterozygous GT and homozygous GG carriers of G276T (P= 0.032) compared to TT carriers, For adiponectin gene polymorphism T45 G, it was associated with Obesity under Co dominant and dominance models (P= 0.046, P= 0.045) respectively. Heterozygous GT and homozygous GG carriers of T45G were related higher BMI (P= 0.029), triglyceride (P= 0.029), and VLDL (P= 0.026) compared to TT carriers at adiponectin polymorphism T45G. Conclusion: The study suggests that the ADIPOQ 276G>T and ADIPOQ 45T>G polymorphisms may be vital indicators of obesity and associated complications in several population.<span class="Apple-converted-space"> </span>https://blj.journals.ekb.eg/article_47614_1f01b01e2c21a38bd04b58021d34ed32.pdf