Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201Assessment of Cytokeratin 17 Levels in Sera of Patients with Invasive Ductal Breast Carcinoma.11114657110.21608/blj.2020.146571ENOthman A.OthmanBiochemistry Division, Faculty of Science, Minia University, Minia, Egypt.Mariana F.GayyedPathology Dept., Faculty of Medicine, Minia University, Minia, Egypt.Fatma S.FathyBiochemistry Division, Faculty of Science, Minia University, Minia, Egypt.HishamIsmailBiochemistry Division, Faculty of Science, Minia University, Minia, Egypt.Journal Article20210103<strong>Background:</strong> Breast cancer (BC) is the most common cancer for women and its incidence is gradually increasing. Here, the serum levels of basal cytokeratin 17 (CK17) and its association with clinicopathological characteristics of patients with invasive ductal breast carcinomas(IDBC) were investigated. <strong>Methods: </strong>Preoperative/pretreatment sera were obtained from 78 female patients with IDBC and sera from 24 age-matched healthy females were used as controls. Serum levels of CK17 were determined using sandwich ELISA. Statistical analysis was performed using SPSS program. <strong>Results: </strong>Serum CK17 levels were significantly higher in IDBC patients (1.26± 0.11; 0.93ng/mL) than controls(0.24± 0.01;0.23ng/mL). Statistical analysis showed that CK17 serum levels significantly correlated with age, menopausal status, tumor size, histologic grad, and pTNM stage of IDBC patients. Moreover, higher mean CK17 levels significantly associated with triple-negative subtype. At the best cut-off level (0.31), the CK17 assay showed area under ROC curve of 0.944 indicating high diagnostic performances.<strong> Conclusion:</strong> The serum CK17 may be a prerequisite marker in invasive ductal breast carcinoma.Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201Molecular mechanism of the anticancer effect of sweet red pepper extract121914657610.21608/blj.2020.146576ENKarim SamyEl-SaidBiochemistry Division, Chemistry Department, Faculty of Science, Tanta University, EgyptEhab MostafaAliBiochemistry Division, Chemistry Department, Faculty of Science, Tanta University, EgyptMaisa MohamedAbd ElhamidBiochemistry Division, Chemistry Department, Faculty of Science, Tanta University, EgyptEl-RefaieKenawyChemistry Department, Faculty of Science, Tanta University, EgyptJournal Article20200107<strong><em>Background: </em></strong>Having non-toxic anticancer agents are targeted by researcher in the biomedical field around the world. Cancer-fighting phytochemicals have been potentially identified in several medicinal plants for cancers therapy. Apoptosis paid a great promise to study mechanism of cancer treatment. <strong><em>Objectives:</em></strong> This study aimed to investigate the antioxidants activities of the aqueous ethanolic extract of sweet red pepper (Capsicum Spp.) and its effects on colorectal cancer cell lines (CaCO-2). <strong><em>Methods:</em></strong> Phytochemical constituents and anticancer efficacy of sweet red pepper extract were evaluated. The apoptotic mechanism of the extract was proved by using real time polymerase chain reaction (RT-PCR) for apoptotic related genes (Bax, caspase 3 and Bcl-2). <strong><em>Results:</em></strong> The results showed that sweet red pepper extracts exhibited powerful antioxidant activities. Moreover, this extract could trigger apoptosis in human colorectal tumor cells by overexpressing apoptotic genes (Bax and caspase 3) and down-expressing the anti-apoptotic Bcl-2 genes. <strong><em>Conclusion:</em></strong> The sweet red pepper extract could inhibit CaCO-2 cellular proliferation by inducing apoptosis due to their phytochemical contents.<strong><em> </em></strong>Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201Anticancer activity and antioxidant potential of Crocus Sativus .L( saffron ) aqueous extract against Ehrlich Ascites Carcinoma cells in Swiss albino mice.204914658410.21608/blj.2020.146584ENFaten Z.MohammedDepartment of chemistry, Branch of Bio chemistry, Faculty of science, Zagazig University, EgyptEhabToussonDepartment of zoology, Faculty of science, Tanta University, EgyptFardousYassinDepartment of chemistry, Branch of Bio chemistry, Faculty of science, Zagazig University, EgyptJournal Article20200123Cancer is considered one of the most common causes of morbidity and mortality worldwide. Many chemopreventive agents have been associated with antiproliferative and apoptotic effects on cancer cells because of their high antioxidant activity. The current study was aimed to evaluate the anti-tumor, antioxidant and anti-inflammatory effects of Saffron aqueous extract and its active components against Ehrlich Ascites tumor (EAC). A total of 120 female mice were randomly divided into eight groups. The antitumor effect was assessed by evaluating tumor volume, tumor cell count , survival time and increase in life span of EAC bearing mice. The effect of Crocus Sativus .L ( saffron ) aqueous extract , Crocin and Safranal on different parameters (liver enzymes , kidney function , cardiac markers and antioxidant parameters). These results indicated that Saffron , Crocin and Safranal is a promising protective agents against EAC cells. <strong>.</strong> Crocus Sativus L ( saffron ) aqueous extract (100mg/kg), Crocin(10 mg/kg) and Safranal(1/10 LD<sub>50</sub>) (0.188 ml/kg) showed significantly decreased (p < 0.0001) in the volume of the EAC and in the count of EAC cells and increase the life span of EAC bearing mice. Also, showed significantly increased in antioxidant levels, decreased the lipid peroxidation( oxidative stress) as compared to positive control group . We found that The treatment of Crocus Sativus . L ( saffron ) aqueous extract , Crocin and Safranal significantly reduced the liver enzymes, cardiac markers and reduced elevated levels of Urea, Uric acid and Creatinine in positive control as compared with negative control and also increase the level of albumin as compared with positive control group . our data was confirmed by histopathological examination.Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201Association between serum level of pentraxin-3 and interleukin-40 with HCC disease in Egypt506214658610.21608/blj.2020.146586ENNoha MohamedSaidBiochemistry Division, Chemistry Department, Faculty of science, Zagazig University, Zagazig, EgyptFathyYassinChemistry Department, Faculty of science, Zagazig University, Zagazig, EgyptAsmma M.EshClinical Pathology Department, Faculty of Medicine ,Zagazig university , Zagazig ,Egypt.EmanSaeedBiochemistry Division, Chemistry Department, Faculty of science, Zagazig University, Zagazig, EgyptJournal Article20200207<em><strong>Background</strong></em><em> Hepatocellular carcinoma (HCC) is a global public health problem that lacks efficient methods for early diagnosis. The inflammation of the hepatocytes due to the cirrhosis caused by HCC can be reflected by the disturbance in the level of some proteins secreted by the inflammatory immune cells. We aimed to study pentraxin-3 (PTX3) and interleukin-40 (IL-40) blood levels in patients with HCC <strong>Material and method </strong>This study included 46 of HCC patients and 24 of control with no history of malignancy. All the volunteers are subjected to complete clinical examination and routine laboratory analysis including CBC, coagulation profile tests, liver and kidney function tests. Serum levels of AFP, interleukin-40, and pentraxin-3 were measured by the ELISA technique <strong>Results</strong> Both PTX3 and IL-40 blood levels were significantly higher in HCC patients compared to healthy controls (</em><em>P</em><em> < 0.05). In addition both of them high AUC under ROC curve with significant value (AUC= 0.97, </em><em>P</em><em> <0.001; AUC=0.745, </em><em>P= </em><em>0.016) for PTX3 and IL-40 respectively. Almost no studies have discussed the role of IL-40, which is new characterized cytokine, with HCC disease. <strong>Conclusions:</strong> This study suggested thatpatients with HCC showed increased presence of PTX3 and IL-40 serum level which may suggest a potential relationship of both of them with HCC. Although the role of IL-40 in HCC pathogenesis and development is still unclear but several studies have revealed that HCC progression is solely dependent on the extent of liver inflammation, hence, the balance between pro-inflammatory and anti-inflammatory cytokines is the key ingredient for controlling the disease progression.</em>Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201Serum Inducible Protein -10 chemokine as a biomarker for clearance of HCV with and without treatment in Egyptian patients637814659010.21608/blj.2020.146590ENFaten Z.MohammedProfessor of Biochemistry, chemistry department, Faculty of Science, Zagazig University, Egypt.Ashraf A.TabllProfessor of Medical Biotechnology, National Research Center, Egypt.ReemElshnawyMedical Biotechnology, National Research Center, Egypt.Hazem S.ElsharkawyB. Sc., Department of Chemistry, Biochemistry division, Faculty of science, Tanta University.Journal Article20200208<strong><em>Background:</em></strong><br /> Hepatitis C virus (HCV) is a major health problem worldwide particularly in Egypt. Chemokine IP-10 may be a good prognostic marker for the outcome of HCV treatment<br /> <strong><em>Aim:</em></strong><br /> Assessment the potential predictive value of Serum Inducible Protein -10 chemokine (IP-10) in the clearance of HCV RNA in Egyptian patients with and without treatment<br /> <strong><em>Materials and Methods: </em></strong>Ninety Egyptian individuals were involved in the current study where, 20 (23%) patients were chronic HCV chronic (positive HCV antibodies and positive HCV RNA without treatment, 20 (22%) were healthy individuals (negative for both HCV antibodies and HCV RNA, 20 cases (22%) were natural clearance (positive HCV antibodies and negative for HCV RNA without treatment), 20 (22%) were achieved SVR after treatment (responder group, HCV positive and negative for HCV RNA after treatment) and 10 (11%) were non responder (positive HCV antibodies and still positive HCV RNA after treatment. HCV RNA was quantitated by real time PCR for and serum IP10 level was measured by commercial ELISA kit. All biochemical and hematological examinations included liver function, CBC and alphefeto protein were assessed.<br /> <strong><em>Results:</em></strong> The mean serum levels of IP-10 were significantly higher (p < 0.001) in CHC patients (345.4±100) pg/ml than healthy control group (101.5±31.4) and natural clearance group (103.2±40.7). Also serum levels of IP-10 was significantly elevated in non responder group (257.4±52.5) compared with each of SVR group (103.5± 43.5) (p < 0.001) and healthy group (101.5±31.4), (p < 0.001). Prediction of a clinical response based on a combination of these chemokines revealed high sensitivity (82%), specificity (85%), negative predictive value (95%), and area under the curve (1.00). Moreover, there is no correlation ((R= 0.05), P value p < 0.795) between serum level of IP-10 and HCV viral load.<br /> <strong><em>Conclusion</em></strong>: IP10 is a useful non-invasive biomarker for viral clearance and might be used to apply patients according to the predictable treatment outcome. Accordingly, patients who are unlikely to respond to treatment would avoid unnecessary exposure to medication that is related with high morbidity.<br /> .Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201In vitro and in vivo studies on the anticancer potential of curcumin and nanocurcumin798914659210.21608/blj.2020.146592ENLamiaa A.A.BarakatAssissant prof of Biochemistry,chemistry department, portSaid UniversityJournal Article20200229Purpose: Curcumin, a polyphenolic compound that obtained from the herb of Curcuma longa, has many anticancer effects. But, its effect is low due to poor water solubility. In order to improve its solubility and drug delivery, we have utilized a nano-curcumin. Methods: In vitro cytotoxicity and anti-tumor promoting effects of nanocurcumin and normal curcumin were investigated. Results revealed that nanocurcumin is able to inhibit the growth of two human cancer cell lines Hep-G2 and HCT116 with IC50 values of 5.68 and 6.53 µg ml−1, respectively,while free curcumin expresses the activity with IC50 values of 8.28 and 9.64µg ml−1. At the concentration of 40 µg ml−1. Nanocurcumin showed anti-tumor promoting effects in reducing tumor size by 59.8 % , while the percentages caused by curcumin was 41.4%, respectively. Mice were treated with equal concentration of nanocurcumin and curcumin showed an improvement in the antioxidant and anti-inflammatory cytokines. The level of improment in EC bearing mice treated with curcumin was lower than that of cells treated with nano curcumin (P=0.001). Conclusion:Results are suggesting that Nano curcumin can be more effective than free curcumin in inhibition of Elrich ascites carcinoma cell lines.Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201The effect of "3, 6 diamino-5-cyano-4-(p-chloro phenyl) - Pyrazolo [3, 4- b] pyridine” on Ehrlich ascites carcinoma in mice9010014659510.21608/blj.2020.146595ENMohamed E.A.HassanDepartment of bioChemistry, Faculty of Science, Zagazig University, EgyptHaytham AAliDepartment Biochemistry Faculty of Vet medicine Zagazig UniversityWaiel M.Salah El DienAnimal Health Research Institute, Zagazig Prov.Lab., Dep.of Food HygieneAtef M.AmerDepartment of Chemistry, Faculty of Science, Zagazig University, EgyptJournal Article20200308Pyrazolopyridine derivatives are organic substance with novel properties. They have many beneficial uses as antibacterial and antifungal etc; so this study was designed to investigate the antitumor and hepatoprotective effect of "3, 6 diamino-5-cyano-4-(p-chloro phenyl) - Pyrazolo [3, 4- b] pyridine" in comparison to folfox (5-flouro uracil 50 mg/kg &6mg/kg oxaloplatine). Thirty five adult male mice (24-26 g) was divided into five groups (7 each).first one considered as a negative control, second as positive control, third and fourth treated intraperitoneal with 35 mg/kg B.WT. Of compound from the 1<sup>st</sup> day and after one week of EAC injected respectively. Serum analysis of liver enzymes showed a significant increase in ALT, AST and significant decrease in serum Albumin and total protein activity, also there were an elevation in inflammatory gene expression of cytokine TNF-alpha and insignificant elevation in gene expression of CDKn2D. and after treatment, the liver function showed normal level and significant decreases of gene expression of cytokine TNF-alpha. In addition, gene expression of CDKn2D showed significant elevation in it is level . Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201The effects of weight loss intervention program on serum leptin level, histological and molecular alterations of adipose tissue in rat model with obesity.10110814659810.21608/blj.2020.146598ENNearmeen M.RashadInternal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.Sally E.SayedBiochemistry Department, Faculty of Science, Zagazig University, Zagazig, EgyptMohamed H.SherifOrganic Chemistry Department, Faculty of Science Zagazig University, Zagazig, Egypt.Mahmoud Z.SitohyBiochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig Egypt.Journal Article20200320Background: Obesity is a resilient and complex chronic disease.<br /> Leptin is an adipocyte-derived hormone acts as a key regulator of feeding and energy expenditure.<br /> The aim of our study was to assess the level of serum leptin as well as leptin immuno-histochemistry expression in adipose tissue in obese and non-obese rats.<br /> Leptin was markedly detected in young preadipocytes during lipogenesis (before weight loss (regime) in contrast to adipocytes undergoing lipolysis (after weight loss (regime) in the later weak positive.<br /> <br /> Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201Impact of gene polymorphism on Egyptian HCV patients under direct antiviral Drugs10911914660410.21608/blj.2020.146604ENSoha M.HamdyProfessor of Bio chemistry, Chemistry Department, Faculty of Science, Fayoum UniversitySara H. A.AgwaAssistant consultant of clinical pathology& molecular biology in the HCV research and treatment unit in Faculty of Medicine, Medical Ain Shams Research Institute (MASRI), Cairo-EgyptAhmed A. G.AfifyProfessor of Tropical Medicine, Gastroenterology Department, Faculty of Medicine, Fayoum UniversityAmany M.MohmmedAssistant Professor of Biochemistry, Chemistry Department, Faculty of Science, Fayoum UniversityBasma M. M.AliDemonstrator of Biochemistry, Chemistry Department, Faculty of Science, Fayoum UniversityJournal Article20200407Hepatitis C viral infection is with the highest prevalence rate in Egypt. Direct Acting Antivirals (DAAs) is now the standard of care for HCV infection treatment. Here we assess the predictive value of single-nucleotide polymorphisms (SNP) rs2596542 C/T in chromosome 6 located in MHC class I on the response to DAAs in chronic HCV infected Egyptian patients. This study was performed on 70 Egyptian patients positive for HCV; classified into two groups. Group I: (33 patients) were received combination therapy Sofosbuvir (Sovaldi) 400 mg/day plus Declatasvir 60 mg once daily, Group II: (37 patients) were received Ombitasvir 25 mg, Paritaprevir 150 mg and Ritonavir 100 mg/day plus Ribavirin 15 mg/kg/day for 12 weeks. HCV level by (RT-PCR), MICA single nucleotide polymorphism of rs2596542, ALT, AST, total bilirubin, serum albumin, fasting blood sugar, HbA1C %, CBC, serum creatinine, AFP were performed in all volunteer patients. Results showed that group II responded with 100% in CT- SNP genotype. However both CC and TT- SNP genotypes responded with 83.33% and 91.67% respectively. There was no observed significant association between SNP rs2596542 C/T and all clinical parameters except AFP that give positive significant correlation in the CC genotype.Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201Effect of isolated mesenchymal stem cells on the liver injury of rats12012714660810.21608/blj.2020.146608ENMohga M.Al-EssawyUrology and Nephrology Center, Mansoura University, Mansoura, EgyptMahmoud M.GabrUrology and Nephrology Center, Mansoura University, Mansoura, EgyptMai M.El-KeiyDepartment of Biochemistry, Faculty of Science, Tanta University, Tanta, EgyptTarek M.MohamedDepartment of Biochemistry, Faculty of Science, Tanta University, Tanta, EgyptJournal Article20200413Background: This study aimed to evaluate the ability of mesenchymal stem to treat liver injury. Liver transplantation and surgical treatment may be one of the good available solutions for liver injuries. However, it is painful and limited due to shortage of donor organs and high medical costs. Mesenchymal stem cells (MSCs) can serve as an autologous treatment to the liver injury. This study aims to evaluate the ability of MSCs to treat liver injury. Rat MSCs were isolated, expanded for 4 passages. Then they were injected into the tail vein of Sprague Dawley rats which were previously inducted to liver fibrosis with carbon tetrachloride (CCL<sub>4</sub>). After and before cell injection, the biochemical analysis of liver function tests and the immunohistochemistry were tested for the liver tissue after 6 weeks of disease induction. Conclusion: AD-MSCs have a promising effect against CCL4 induced liver fibrosis as well as enhancing liver function tests.Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201Association between AXIN1 gene polymorphisms (wnt signaling pathway gene) and nephropathy induced by diabetes and hypertension in the Egyptian population12815014661110.21608/blj.2020.146611ENNoha MohamedSaidBiochemistry Division, Chemistry Department, Faculty of Science, Zagazig University, Zagazig, EgyptHamed YousefMoustafaChemistry Department, Faculty of Science, Zagazig University, Zagazig, EgyptSamahAbdel AzizBiochemistry Division, Chemistry Department, Faculty of Science, Zagazig University, Zagazig, EgyptJournal Article20200615<strong><em>Background:</em></strong> Chronic renal disease is a public health concern worldwide. The epidemiology of this disease can help in the protection strategy. The detection of the polymorphism of the CKD candidate gene can assist in the early detection Chronic. And thus, early detection and treatment can often avoid or postpone the kidney disease. <strong><em>Objectives:</em></strong> Our research aimed to investigate how polymorphisms of the AXIN-1 gene and CKD incidence are associated with Egyptians. <strong><em>Methods:</em></strong> <em>This study included 3 groups; control group, a pre-nephropathy group including (diabetes 2 and hypertension) subgroups, and a nephropathy group including (diabetic nephropathy, hypertensive nephropathy, and diabetic hypertensive nephropathy) subgroups. All the volunteers are subjected to complete clinical examination and routine laboratory analysis.</em> <em>In this research, we performed genotyping for two SNPs (rs9921222 and rs1805105) throughout the Egyptian population (PCR-RFLP). </em><strong>Results</strong>: our results showed that in both <em>(rs9921222 and rs1805105) SNPs, there was a significant difference in genotypes distribution (P</em><em><0.001)</em><strong><em> Conclusion:</em></strong> The AXIN-1 SNPs <em>(rs9921222 and rs1805105) </em>may be used as one of the Egyptian population's CKD susceptibility factors and larger studies can confirm our results.Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201Telomerase Reverse Transcriptase and miR-34a in diagnosis of non-muscle invasive bladder cancer15115914661610.21608/blj.2020.146616ENWaleed YousefAbd El- RazekDepartment of Chemistry, Faculty of science,Menoufia University, Menoufia, Egypt.AmiraAwadallaCenter of Excellence for Genome and Cancer Research, Urology and NephrologyM.A.ElbasetCenter of Excellence for Genome and Cancer Research, Urology and NephrologyAhmed El-SayedAbdel-MegiedDepartment of Chemistry, Faculty of science,Menoufia University, Menoufia, Egypt.Faten ZahranMohamedDepartment of Chemistry, Faculty of science, Zagazig University, Zagazig, Egypt.Ahmed A.ShokierCenter of Excellence for Genome and Cancer Research, Urology and Nephrology.Journal Article20200710<strong>Background: </strong>Management of high-risk non-muscle invasive bladder cancer is difficult, as no validated tool exists to predict risk of recurrence. Urine has the potential to contain a variety of molecular markers that may be associated with tumors.<br /> <strong>Methods:</strong> The study included 3 groups: group 1comprised 100 patients diagnosed with non-muscle invasive bladder cancer<strong> (</strong>NMIBC). Each of Group 2 included 100 patients with other pathology rather than NMIBC. Group3 comprised 100 healthy persons. Group1 was subdivided into patients with and without recurrence. The patients with recurrent tumors were subclassified into single or multiple recurrences. Urinary markers: TERTand miR-34a were evaluated.<br /> <strong>Results:</strong> Higher urinary TERT > 3.2 and lower urinary miR34a < 0.85 were detected significantly in NMIBC group compared to controls.<br /> <strong>Conclusion:</strong> Urinary molecular biomarkers are reliable non-invasive tools for NMIBC detection and prediction of recurrence. Urinary TERT >3.2 and miR34a< 0.85 were significantly higher in patients with NMIBC in comparison with controls. Urinary TERT > 3.2 had the highest overall diagnostic accuracy for bladder cancer detection.Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201Effect of Selenium nanoparticles in wound healing16016814661710.21608/blj.2020.146617ENKeshtaA.T.Assistant professor of Biochemistry, Chemistry Department, Faculty of Science, Zagazig universityEmamMBiochemistry division, Chemistry Department, faculty of Science, Zagazig Univeristy.AttiaY.AAssistant professor of Physical chemistry, National Institute of Laser Enhanced Sciences, Cairo UniversityJournal Article20200910<strong>Background:</strong> Wound healing is a complex process necessary to repairing damaged tissues and preventing infection. Selenium nanoparticles were known by their antioxidant and antimicrobial effect that is important in wound healing. <strong>Aim:</strong> the aim of this study was to investigate the effect of selenium nanoparticles in reducing and accelerating the wound healing time in mice. <strong>Methods:</strong> Se NPs were synthesized by a simple wet chemical method. Then the experimental animals were divided into the following groups: Negative control group, Positive control group, selenium nanoparticles control group, wounded mice treated with selenium nanoparticles group. At the end of experiment, mice were scarified& skin tissue samples were collected for biological analysis [Vascular Endothelial cell Growth Factor, Collagenase I and Nitric oxide] and histopathological study. <strong>Results:</strong> The results showed that the percentage of wound area has been significantly reduced in Se NPs treated group compared to the positive control. The level of Vascular Endothelial Growth Factor and Collagenase I in Se NPs groups were significantly increased compared to positive control while nitric oxide was significantly decreased in Se NPs treated group. Skin tissue showed effect of selenium nanoparticles by regenerating and recavering skin layer. <strong>Conclusion:</strong> Selenium nanoparticles have an important role in accelerating and reducing wound healing time.Zagazig University; Faculty of Science. Center of Scientific Studies and ResearchesBiochemistry Letters1687-477316120201201Anti- diabetic activity of new Synthesized Flavonoid compound16918425450310.21608/blj.2020.254503ENAl-ShimaaM. AbasBiochemistry Division, Chemistry Department, Faculty of Science, Zagazig University, EgyptFawziaZ. El-AblackOrganic Chemistry, Chemistry Department, Faculty of Science, Damietta University, new Damietta 34517, EgyptJournal Article20220813<strong>Background:</strong> Diabetes mellitus (DM) is associated with long-term damage, dysfunction, of various organs. <strong>Objectives</strong>: Study aims to assess the role of new Synthesized Flavonoid compound on experimentally induced diabetes. <strong>Methods</strong>: 50 adult male albino rats divided into 5 groups. Group 1 (control group, rats were orally administered with 1 ml saline daily). Group 2 (DMSO group, rats were orally administered with 0.2 % DMSO for 60 day orally). Group 3 (positive control, animals were injected intraperitoneally with 60 mg/kg b.wt streptozotocin followed by intraperitoneal injection with 120 mg/kg b.wt of Nicotinamide after 15 minute).Group 4 (standard group, diabetic animals treated with 100 mg/kg b.wt of metformin for 60 day orally). Group 5 (therapeutic group, diabetic rats treated with 50 mg /kg b.wt of Ethyl 2-amino-4-phenyl-4H-benzo(h)chromene-3-carboxylate for 60 day orally). At the end of experimental period blood serum & plasma samples, liver, kidney and pancreatic tissues were collected. <strong>Results:</strong> diabetic rats showed significant increase in plasma glucose, serum urea, creatinine, cholesterol and triglyceride . Also significant increase in mean level of Fetuin A and Netrin-1 in serum and different organs (Liver, kidney, Pancreas) in compared to control group. Oral administration of Ethyl 2-amino-4-phenyl-4H-benzo(h)chromene-3-carboxylate cause decrease in elevated biochemical parameters. Also, decrease Fetuin A and Netrin-1 levels when compared with diabetic rats. Molecular docking studies confirmed binding of compound with Fetuin A and Netrin-1 proteins in terms of energy and revealed of the existence of hydrogen bond ,hydrophobic interaction,Our results were confirmed by histopathological examination of pancreatic tissue. <strong>Conclusion:</strong> this study suggests that Ethyl 2-amino-4-phenyl-4H-benzo(h)chromene-3-carboxylate exihibits antihyperglycemic activity in streptozotocin- induced diabetic rats.