The effect of "3, 6 diamino-5-cyano-4-(p-chloro phenyl) - Pyrazolo [3, 4- b] pyridine” on Ehrlich ascites carcinoma in mice

Document Type : Original Article

Authors

1 Department of bioChemistry, Faculty of Science, Zagazig University, Egypt

2 Department Biochemistry Faculty of Vet medicine Zagazig University

3 Animal Health Research Institute, Zagazig Prov.Lab., Dep.of Food Hygiene

4 Department of Chemistry, Faculty of Science, Zagazig University, Egypt

Abstract

Pyrazolopyridine derivatives are organic substance with novel properties. They have many beneficial uses as antibacterial and antifungal etc; so this study was designed to investigate the antitumor and hepatoprotective effect of "3, 6 diamino-5-cyano-4-(p-chloro phenyl) - Pyrazolo [3, 4- b] pyridine" in comparison to folfox (5-flouro uracil 50 mg/kg &6mg/kg oxaloplatine). Thirty five adult male mice (24-26 g) was divided into five groups (7 each).first one considered as a negative control, second as positive control, third and fourth treated intraperitoneal with 35 mg/kg B.WT. Of compound from the 1st day and after one week of EAC injected respectively. Serum analysis of liver enzymes showed a significant increase in ALT, AST and significant decrease in serum Albumin and total protein activity, also there were an elevation in inflammatory gene expression of cytokine TNF-alpha and insignificant elevation in gene expression of CDKn2D. and after treatment, the liver function showed normal level and significant decreases of gene expression of cytokine TNF-alpha. In addition, gene expression of CDKn2D showed significant elevation in it is level           .         

Keywords

Files

Share

How to cite

Statistics