Rapamycin and Filgrastim effect on Regulation of Peripheral and Splenic Tissue B- and T-lymphocytes in TAA-Induced Splenic Injury in Rats

Document Type : Original Article

Authors

1 Biochemistry division, Chemistry Department, faculty of Science, Zagazig University.

2 Assistant professor, Medical Histology and cell biology Department, Faculty of Medicine, Zagazig University.

Abstract

Background: Splenomegaly; a bigger spleen pool for
blood, affecting circulating and its resident blood cells.
Aim: this study aimed to evaluate the effects of
Rapamycin (RAPA) and Filgrastim (FIL) treatment
separately and in combination on the blood and splenic
lymphocytes in thioacetamide (TAA)-induced splenic
injury. Methods: Thirty-six adult male albino rats were
divided into six groups; negative control, TAA-treated,
TAA+RAPA-group,TAA+FIL-group, TAA+RAPA+FILgroup < /p>
and TAA+RAPA then FIL-group. For each group,
complete blood count (CBC), flow cytometry for blood
CD3 and CD19 lymphocytes, and immunohistochemical
detection of splenic CD3 and CD20 positive lymphocytes
were performed. Results: the results demonstrated
significant increased blood CD3 and CD19 lymphocytes
with increased splenic CD3 and CD20 lymphocytes,
decreased Red blood cells (RBCs), Hemoglobin (Hb) and
increased White blood cells (WBCs) with marked effect
on spleen morphology after TAA injection. RAPA
treatment showed decreased splenic lymphocytes while
increased blood T-cells with increased Hb levels and
granulocytes percentage, lowered WBCs count with more
decreased lymphocytes percentage and further reduced
Platelets (Plts) count. Also, FIL monotherapy showed
decreased splenic lymphocytes with decrease in
circulating WBCs with increased Hb levels. Both
combination treatments caused an increment in blood
CD3 lymphocytes, while only decreased CD19
lymphocytes with TAA+RAPA+FIL-group. Conclusion:
Splenic and blood lymphocytes were affected by RAPA
and FIL separately or in combination as combination
showed a synergistic effect on spleen lymphocytes.

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