Immune response of Enterobacter aerogenes ghost vaccine in mice

Document Type : Original Article

Authors

1 Department of Chemistry (Biochemistry Division, Faculty of Science, Zagazig University, Egypt

2 Department of Immunology and Allergy, Medical Research Institute, Alexandria University, Egypt

3 Department of Protein Research, City of Scientific Research and Technological Applications, Alexandria, Egypt

4 Department of Clinical Biochemistry, Faculty of Medicine, King Khalid University, Abha, KSA.

5 Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Egypt

Abstract

Background: The emergence of multidrug-resistant Enterobacter aerogenes has become a public health concern. E. aerogenes is a common cause of hospital-acquired infections such as pneumonia and urinary tract infections. Aim: This study focused on the use of E. aerogenes ghosts as a potential vaccine against the opportunistic pathogen E. aerogenes. Methods: This study used a cost-effective method to generate the E. aerogenes ghosts using certain chemicals. The safety and effectiveness of the ghosts were evaluated in a mouse model by administering three doses over a 14-day period. The mice were then challenged with a live E. aerogenes strain. Results: The results of the cytokine assays showed that vaccinated mice produced higher amounts of IL-10 and TNF-α than did control saline mice, indicating a good immune response. Additionally, the results of the liver function tests indicated that there was a decrease in the activities of ALT and AST in the vaccinated mice as compared to the non-vaccinated mice and low levels of TP and Alb in non-vaccinated mice, suggesting a reduced liver injury and inflammation in vaccinated mice. Conclusion: Overall, the study suggests that E. aerogenes ghosts may be a promising candidate for use as a vaccine against E. aerogenes infection.

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