Antioxidant activity of curde exopolysaccharides from marine bacteria

Document Type : Original Article

Authors

1 biochemistry &chemistry,science,zagazig

2 Department of Chemistry, Faculty of science, Zagazig University. Egypt

3 botany and microbiology Depatment,faculty of science,benha university

4 Microbial biotechnology dept.National research centre

5 Chemistry department, Faculty of science, Zagazig university, Zagazig, Egypt

Abstract

Marine microorganisms often produce bioactive substances with novel functions and structures because of their special living conditions. Polysaccharides from marine microorganisms are of interest to new drug discovery. Twenty bacterial isolates were isolated from Red Sea. These isolates were screened for production of exopolysaccharides (EPSs), the maximun bacterial isolate was (RS10) which proudced (8.84 g/l) from (4.03 g/l). Therefore, there are four isolates have no the ability to produce EPS (RS6, RS11, RS13 and RS19). Eleven EPSs were screened for antioxident activity by determination of DPPH free radical scavenging activity, the highest one was RS10 (95.85 %) at 90 min. while, the lowest one was RS5 (36.70 %) at 90 min. By study the effect of some factors on the production of EPSs by isolate (RS10), Thus maximam EPS yield was (11.2 g/l) and cell dry was (4.6 g/l) by Incubation temperature 40°C after three days, RPM 120, pH 7, beef extract as nitrogen sources and sucrose with (20 g/l) as carbon source.

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