Document Type : Original Article
Authors
1
Department of Chemistry, Biochemistry Division, Faculty of Science, Fayoum University, El-Fayoum, Egypt
2
professor of Tissue Culture and Regenerative Medicine Medical Ain Shams Research Institute Faculty of Medicine, Ain Shams University,Cairo,Egyp
3
Fellow of molecular biology and tissue culture in MASRI, Faculty of medicine,Ain sham university,Cairo,Egypt
4
Zoology Department, Faculty of Science, Fayoum University, Fayoum, Egypt
Abstract
Objective: The objective of our study is to evaluate the therapeutic effect of Bone marrow mesenchymal stem cells (BMMSCs) on the hepatotoxicity induced by carbon tetrachloride(CCL4) relative to Curcumin(Cur) as a treatment derived from herbal plants. Material & Methods: Thirty-five adult male Western Albino Rats were used, were divided into four groups. group(I) : Control gp ,group (II): CCL4 gp ,group (III) : Cur treated gp , and group (IV): BMMSCs treated gp . Isolation and preparation of BMMSCs, detection of cell-surface markers by flow cytometry, blood samples were collected which in turn used to analyse liver function tests: glutamate oxaloacetate transaminase (SGOT), glutamate pyruvate transaminase (SGPT), alkaline phosphate (ALP), total bilirubin, total protein (TP), total antioxidant (TAC) , malondialdehyde (MDA) ,Hemoglobin concentration (HB), White blood cells) WBCs( count and Platelet (PLT) count . Histopathology and immunohistochemistry were also performed. Results: The liver damage that resulted from CCl4 showed a significant rise in (ALT, AST , ALP, MDA, T. bilirubin, WBCs count, as well as a decrease in T.P , TAC, HB concentration, and PLT count. treatment with BM- MSCs enhanced liver state more than curcumin ,. It elevated the levels of T.P, TAC, HB, and PLT while considerably lowering the levels of AST, ALT, ALP, MDA, T.bilirubin, and WBCs. Histopathological was marked BM-MSCs improved liver construction. Conclusions:Curcumin and BM-MSCs improve liver function and lessen the toxicity by restoring liver structure and function in a rat model of CCL4-induced hepatotoxicity ,but treatment with BM-MSCs improved liver condition more successfully.
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