Anti-inflammatory response of Metformin and/or low doses of γ-Irradiation on Acute Pancreatitis induced by L-arginine in Rats

Document Type : Original Article

Authors

1 Biochemistry Department, Faculty of Science, Zagazig university

2 Radiation Biology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority.

3 Chemistry department, Faculty of science, Zagazig university, Zagazig, Egypt

Abstract

Pancreatitis is an inflammatory disorder of the pancreas. Objectives: The purpose of the current study was to assess the anti-inflammatory properties of metformin (Met) and/ in combined with low doses of γ-irradiation (IR) against acute pancreatitis (AP) caused by L-arginine in an animal model. Methods: From among thirty adult Wister rats, five groups (n=6) were randomly selected. Normal control; AP rats; AP rats exposed to IR; AP rats treated with Met, and AP rats treated with Met and exposed to IR. One week following the end of experimental treatments, microscopic examination on pancreatic tissue and biochemical parameters were measured. Results: AP was evidenced by elevating the serum pancreatic enzymes (amylase & lipase) levels. a significant increase in pro-inflammatory markers (IL-6, IL-8, TNF-α, RBS, and CRP (, and a significant decrease in anti-inflammatory marker (IL-10), insulin levels, and Insulin growth factor 1 (IGF1) were detected. Microscopic examination revealed that L-arg. treated animals showed a cluster of immune cells, lymphoid aggregates, hemorrhage, necrotic acini and detachment of lobules. All the serological parameters and the histopathological observations were markedly improved by Met and/or low doses of γ-irradiation treatment either alone or combined. Conclusion: Met and/or low doses of IR could have a therapeutic effect on the acute pancreatitis model induced in rats.

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