Document Type : Original Article
Authors
1
professor of clinical immunology, Head of center MARC-CSC, Faculty of Medicine, Mansoura University, Egypt.
2
Professor of Biochemistry, chemistry department, Faculty of Science, Zagazig University, Egypt.
3
Lecturer of clinical pathology, Faculty of Medicine, Mansoura University, Egypt.
4
Mansoura University Children Hospital, faculty of medicine, Mansoura University.
Abstract
Background: Stem cells from umbilical cord blood Lifetime protection umbilical cord blood contains stem cells. These stem cells were until recently received and discarded after delivery with umbilical cord and placenta. However, these cells are of great value at the moment of birth and kept for life for any use. May be necessitated by urgent medical imperatives given later. Human stem cells derived from the umbilical cord have many advantages: they are easily collected without harm to the child or mother and treatment of many diseases including blood diseases, cancer and immune system disorders.Because of the high cost of treatment and preservation of umbilical cord blood and the relative proportion of stem cells in which the banks of stem cells from umbilical cord blood paid great attention to the acquisition of high-quality units to ensure higher rates of success of stem cell transplantation and so I study the factors that may affect the quality of umbilical cord blood units is important. Aim: To determine the characteristics of the laboratory hematopoietic potentiality of umbilical cord blood hematopoietic stem cells, and to study their association with maternal and neonatal factors. Materials & Methods: The maternal and neonatal factors that influence the total nucleated cell (TNC), and CFU yields in CB collected for the Cord Blood Bank were evaluated.Results: As expected, there was a significant rise in TNC / CBU and CFU / CBU considering quality of UCB unit with increase in UCB volume, and also there was significant elevation of cord blood volume, TNC x 108 / CBU and CFU / CBU x105 in units collected from birth weight ˃ 3500 gm compared to that of birth weight ≤ 3500 gm. Fetal gender affected quality of UCB units, Volume of UCB units in our study was significantly higher in male fetus compared to only in female fetus. TNC x 108 / CBU was elevated in preterm than collected from post term. CFU / CBU x 105 in preterm compared to term and post term. The increase in maternal age was associated with high volume, TNC and CFU. There is significant elevation of CBU volume, TNC , from maternal age > 20 years compared to that of maternal age ≤ 20 years. Conclusions: Our study suggests that maternal donor characteristics significantly influence the yield of TNC and viability of UCB samples. These factors should be considered when attempting to improve the yield of potential stem cells in cord blood
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