Insulin sensitization via PPAR γ and glucose uptake through activation of PI3K/p-Akt signaling pathway by resveratrol in type 2 diabetic rats

Document Type : Original Article

Authors

1 Chemistry Department, Faculty of science, Zagazig University, Zagazig, Egypt

2 Genetic Engineering and Biotechnology Institute, University of Sadat City, Egypt

Abstract

Background: Increasing evidence in both experimental and clinical studies suggests that there is a close link between hyperglycemia, oxidative stress, and diabetic complications. Resveratrol a polyphenolic compound found in various natural food products. Aim: The aim of the present study was to screen insulin sensitization via PPARγ and glucose uptake through activation of PI3K/p- Akt signaling pathway by resveratrol in type 2 diabetic rats Material and methods: Four groups male adult albino rats were used in this study: Negative control, Positive control, Therapeutic group and Standard group. Results: The treatment of resveratrol significantly reduced the elevated levels of blood glucose, Total cholesterol, Triglyceride, LDL-c, Urea and Creatinine in positive control as compared with negative control. Treatment of resveratrol also reverted back the decreased levels of insulin, HDL-c, Irisin, enzymatic antioxidant (catalase and superoxide dismutase) as well as decreased level of the non-enzymatic antioxidant (reduced glutathione) in tissue extracts from different organs of diabetic rats to near normal values. Resveratrol significantly increased expression of the PPAR γ gene in adipose tissue compared to positive control. It also  regulated insulin mediated glucose uptake in adipose tissue through activation of PI3K/p-Akt signaling pathway. PPARγ gene showed many different expressions for other side the PPARγ sequences from the adipose tissue confirm there is a mutation with the gene isolated from positive control against therapeutic group, meaning the Type 2 diabetes cause a mutation in the gene sequencing compared to negative control. Hence, we find the opposite with the PPARγ sequencing in therapeutic group. Conclusion: These results clearly suggest that the resveratrol has an antioxidant effects and could improve adipose tissue insulin sensitivity and maintain glucose homeostasis in adipose tissue. 

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