A series of experiments was designed to investigate the activity of the SV40 early promoter and enhancer in rat pituitary tumor cells in culture but still yet not clear so, a series of enhancer promoter-reporter plasmids were constructed to study the SV40 activity in the rat pituitary tumor cell lines (GH3 and GC) then sub-cloned into pSV2(enhancer-minus)-CAT, then purified in gel and ligated into pRSV(enhancer-minus)-CAT (Sph1 restricted) then transformed into E.Coli then make restriction analysis. From the results we observed that SV40 early promoter can be activated by heterogeneous enhancers in pituitary tumor cells.
El-jaffry, S., Fehalbum, I., A. A, H., & Hussein, M. M. (2013). The transcriptional activity of the simian virus 40 enhancer and promoter in GH3 cells. Biochemistry Letters, 9(1), 1-5. doi: 10.21608/blj.2013.63403
MLA
Salem El-jaffry; I. Fehalbum; Haytham. A. A; Mohamed M.A. Hussein. "The transcriptional activity of the simian virus 40 enhancer and promoter in GH3 cells", Biochemistry Letters, 9, 1, 2013, 1-5. doi: 10.21608/blj.2013.63403
HARVARD
El-jaffry, S., Fehalbum, I., A. A, H., Hussein, M. M. (2013). 'The transcriptional activity of the simian virus 40 enhancer and promoter in GH3 cells', Biochemistry Letters, 9(1), pp. 1-5. doi: 10.21608/blj.2013.63403
VANCOUVER
El-jaffry, S., Fehalbum, I., A. A, H., Hussein, M. M. The transcriptional activity of the simian virus 40 enhancer and promoter in GH3 cells. Biochemistry Letters, 2013; 9(1): 1-5. doi: 10.21608/blj.2013.63403
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