INTERLEUKIN-6 PROMOTER POLYMORPHISM (-174 G/C) IN EGYPTIAN PATIENTS WITH BEHCET's DISEASE

Document Type : Original Article

Authors

1 Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Menofiya University, Egypt

2 Department of Rheumatology and Rehabilitation، Faculty of Medicine, Cairo University, Egypt

3 Biochemistry Department, National Liver Institute (NLI), Menofiya University, Egypt.

Abstract

Behçet’s disease (BD) is a multisystem inflammatory disease
characterized by recurrent orogenital ulcerations, ocular
inflammations, and skin lesions. The etiology of the disease is
currently unknown but evidences suggested that there is a strong
genetic component mediating the chronicity of the disorder.
Cytokines seem to have important roles in the pathogenesis of BD.
Its production could be affected by genetic polymorphisms. Thus,
this study aimed at investigating the associations between BD in
Egyptian population and IL-6 (-174) promoter polymorphism. We
genotyped IL-6 (-174) position in the promoter region of IL-6 using
Mutagenically Separated PCR (MS-PCR) in 61 Egyptian patients
with BD and 97 healthy-matched control. No significant association
between IL-6 (-174) polymorphism and BD was found. Only two
genotypes were seen in the gel GC and GG there was no CC in BD
or control. No significant association between IL-6 (-174 G/C)
polymorphisms and BD was demonstrated. Moreover, there was no
significant difference between G or C allele distribution between BD
and controls. Comparing patients with various disease
manifestations, we found no significant association with the -174
G/C genotypes. A remarkable increase (even not statistically
significant) in the frequency of IL-6 -174 GG (92.6%) in BD patients
in active state as compared to BD inactive (73.5%) was found. In
conclusion, our data revealed that IL-6 -174 promoter
polymorphism does not contribute significantly to BD disease
susceptibility or to distinct clinical feature.

Files

Share

How to cite

Statistics