ISOLATION AND CHARACTERIZATION OF ADULT STEM CELLS FROM BONE MARROW

Authors

1 Biochemistry Division, Dept. of Chemistry, Faculty of Science, Zagazig University

2 Faculty of Science, Port said University

3 Faculty of Medicine, Mansoura University.

Abstract

Bone marrow is considered one of the most widely used sources for
obtaining stem cells. The ease of techniques, absence of ethical
dilemmas makes it one favorable source for regenerative medicine
trials. In the present study we isolated MSCs from the bone marrow
of Sprague Dawley rats. Characterization of the isolated cells
revealed their positive CD29, 90 (Mesenchymal stem cells) and
negative CD 45 denoting their mesenchymal lineage and the
supporting the possibility of isolating MSCs from bone marrow. As
such, this would invite us to use BMSCs for further preclinical and
clinical trials in the area of regenerative medicine.