ISOLATION AND IDENTIFICATION OF ADIPOSE TISSUE-DERIVED STEM CELLS

Authors

1 Biochemistry Division, Dept. of Chemistry, Faculty of Science, Zagazig University

2 Faculty of Science, Port said University

3 Faculty of Medicine, Mansoura University.

Abstract

Stem cells are considered new promising tool for treating many
disorders. However, the difficulties in isolation of stem cells, the
immunorejection problems and the ethical debates about their
sources could slow down the progress in their application in clinical
trials. In the present study we were able to isolate mesenchymal stem
cells from adipose tissue. This would overcome the difficult isolation
from elsewhere, the immunorejection problems (as they can be
isolated from the same patient) and of course the ethical debate as
they will be isolated form a known source after taking conste of the
patient. The characterization of the isolated adipose tissue derived
stem cells (ADSCs) revealed mesenchymal markers (CD29,90) and
absence of Hematopoietic markers (CD45). So, these results support
the capacity of adipose tissue to be an easy source for obtaining
functional stem cells