RELATIONSHIP BETWEEN THE TUMOR NECROSIS FACTOR-Α AND ITS RECEPTOR GENE POLYMORPHISM AND TNF-Α PLASMA LEVELS IN EGYPTIAN RHEUMATOID ARTHRITIS PATIENTS

Authors

1 Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt and Head of Medical Laboratories Department, Faculty of Applied Medical Sciences, Taif University, Taif, KSA .

2 Zoology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.

Abstract

Rheumatoid arthritis (RA) it is a model of multigenic
inflammatory disorder in which tumor necrosis factor-α (TNF-α)
plays an important role. Genetic factors may be implicated in the
susceptibility to disease initiation .Objective: The aim of this study is
to elucidate putative association between the −308 G/A
polymorphism in the promoter region of the TNF-α gene and
196M/R polymorphism of TNFRII genes and susceptibility to
disease onset of RA. Methods: A total of 160 RA patients and a
control group of 40 healthy subjects were available for the study. All
patients fulfilled the American College of Rheumatology revised
criteria for RA, Single-nucleotide polymorphisms (SNPs) at position
−308 of TNF-α and 196 of TNFRII genes were determined using
restriction fragment length polymorphism–polymerase chain
reaction (PCR–RFLP). The A allele of the −308 promoter
polymorphism was more prevalent among the patients. 308GA
genotype of TNF-α is associated with a significantly higher serum
TNF-α level. TNFRII 196R allele more prevalent among the
patients .Our findings suggest that the A allele of TNF-α and
TNFRII 196M/R polymorphism are associated with RA
susceptibility.

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