EFFECT OF ACUTE ARSENIC TRIOXIDE TOXICITY AND ITS TREATMENT ON HSP70 AND CATALASE mRNA IN RENAL TISSUES

Document Type : Original Article

Authors

1 Department of Biochemistry, Faculty of Veterinary Medicine, Benha University (Moshtohor), Egypt.

2 Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Egypt.

Abstract

The induction of heat shock protein (HSP70) in tissues relayed on tissue type, damage induced by stress and metal specific as arsenic trioxide (As2O3) toxicity which considered the biggest inducer of HSP70 which represents biochemical finger prints of exposure and provides protective mechanism against subsequent lethal effect of arsenic trioxide toxicity. Forty adult male albino rats (180 ± 20gm) were segregated into four groups, 10 animals each. First group (control group) rats were injected s/c with single dose of saline. Second group, rats were injected s/c with arsenic trioxide (0.043mM).Third group, rats were injected I/P with 0.7mM of meso 2,3di mercaptosuccinic acid (DMSA). Fourth group, rats were injected s/c with arsenic trioxide (0.043mM) followed by I/P injection with (DMSA) (0.7mM) after 30 minutes of arsenic
trioxide dose. The results revealed significant increase in renal lipid peroxidation (measured as malondialdehyde, MDA) which was associated with a significant decrease in the antioxidant systems such as reduced glutathione (GSH) levels and catalase activity in arsenic trioxide intoxicated group. On the other hand, treatment of rats with DMSA after arsenic trioxide led to a significant decrease in MDA concentration and increase levels of GSH and the activity of catalase when compared with those of arsenic trioxide intoxicated group. Furthermore, total protein and total globulins showed significant decrease in arsenic trioxide intoxicated group than DMSA with arsenic trioxide treated group. At the level of gene expression, we found marked elevation in HSP70 mRNA in arsenic trioxide-intoxicated group while in arsenic trioxide group treated with DMSA its level decreased. Catalase mRNA exhibited a decrease in its level in arsenic trioxide-intoxicated group meanwhile, arsenic trioxide-treated group with DMSA returned catalase mRNA levels to normal. Our results concluded that decreased reduced glutathione concentration and catalase activities in arsenic trioxide intoxicated group were the main inducers for HSP70 mRNA in renal homogenate and DMSA was considered as an effective treatment for acute As2O3 toxicity through amelioration of its oxidative stress especially in the first 30 minutes.

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