Earlier studies had shown an effect of glucocorticoids receptor on Pit 1 activation of rPrl promoter activity Treacy. Therefore, the effect of glucocorticoids on Pit 1 inhibition of SV40 enhancer function was investigated in a series of co-transfection studies into glucocorticoids receptor deficient CV-1 cells. Under identical conditions co-transfection of CMV – Pit 1 activated transcription from prPrl (-1960-+38) – CAT; however, expression of GR in the presence of dexamethasone (10-6M) suppressed this activation. The effect of glucocorticoids on the inhibition of SV40 enhancer activity by Pit 1 was investigated. In co- transfection studies in CV-1 cells (Figure 4) it was shown that GR expression and the provision of glucocorticoids (dexamethasone 10-6M) blocked the ability of Pit 1 to inhibit SV40 enhancer activity. Under the same conditions the ability of Pit 1 to activate the rPrl promoter (-1960/+38) was inhibited.
El-Jaafary, S., EL Fighi, A. B., & Hasan, S. A. .. (2010). EFFECT OF GLUCOCORTICOIDS ON PIT 1 INHIBITION OF SV40 ENHANCER ACTIVITY. Biochemistry Letters, 6(1), 113-118. doi: 10.21608/blj.2010.64438
MLA
S. El-Jaafary; A. B. EL Fighi; S. A .B. Hasan. "EFFECT OF GLUCOCORTICOIDS ON PIT 1 INHIBITION OF SV40 ENHANCER ACTIVITY". Biochemistry Letters, 6, 1, 2010, 113-118. doi: 10.21608/blj.2010.64438
HARVARD
El-Jaafary, S., EL Fighi, A. B., Hasan, S. A. .. (2010). 'EFFECT OF GLUCOCORTICOIDS ON PIT 1 INHIBITION OF SV40 ENHANCER ACTIVITY', Biochemistry Letters, 6(1), pp. 113-118. doi: 10.21608/blj.2010.64438
VANCOUVER
El-Jaafary, S., EL Fighi, A. B., Hasan, S. A. .. EFFECT OF GLUCOCORTICOIDS ON PIT 1 INHIBITION OF SV40 ENHANCER ACTIVITY. Biochemistry Letters, 2010; 6(1): 113-118. doi: 10.21608/blj.2010.64438
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