Document Type : Original Article
Authors
1
Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Egypt.
2
Delta University for Science and Technology, Gamasa, Dakahlia, Egypt
3
Hepatology and Gastroenterology Unit, Internal Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
4
Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt
Abstract
Background & Objectives: Hepatic fibrosis is the extreme extracellular matrix buildup of collagen due to the chronic liver disorders. Progressive liver fibrosis results in cirrhosis and hepatic failure. Nilotinib is considered a 2nd generation tyrosine kinase inhibitor, which exhibited antifibrotic efficacy. Stem cells release cytokines and growth factors which exhibited paracrine-mediated antifibrotic and anti-inflammatory effects in vitro and in vivo. Hence, stem cells conditioned medium (SC-CM) may have an antifibrotic role. This study is designed to explain the association between the oxidative stress markers and the hydroxyproline content in liver fibrosis. Methods: Sixty male wistar rats were distributed into six major groups; control group, CCl4 group, Free conditioned media group, (CCl4 & Nilotinib) group, (CCl4 & stem cells exosomes) group, and (CCl4 & Nilotinib & stem cells exosomes) group. They had an intraperitoneal injection with CCl4 for nine weeks and had treatments of Nilotinib, stem cell exosomes (extracted from Liver mesenchymal stem cells), and a combined treatment of Nilotinib and stem cell exosomes from the 5th week to the 9th week of CCl4 intoxication. Oxidative stress parameters (e.g. MDA, SOD, GSH, CAT, NO, and NOS) and hydroxyproline content were estimated for each group. Results: results showed a significant positive correlation between Hydroxyproline and MDA, NO and NOS. Also, there is a significant negative correlation between Hydroxyproline and GSH, SOD and CAT. Conclusion: There is a significant positive correlation between Hydroxyproline and MDA, NO and NOS. And there is a significant negative correlation between Hydroxyproline and GSH, SOD, CAT.
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