Bovine and rat prolactin gene promoter function by glucocorticoids. It was of interest, therefore to examine the possible inhibition by glucocorticoids of pit l induced activation of transcription from the 3'- rPrl promoter mutant generated for this study. The studies were carried out by co-transfection into glucocorticoids receptor (GR) deficient CV-1 cells both pin1 and (GR) expression vectors and the reporter CAT plasmids with upstream rPrl promoter fragments. The effect of glucocorticoids was examined by the Addition of dexamethasone (10-6 M) to the cultures immediately after transfection. In these studies glucocorticoids receptor (GR) deficient CV-l cells were used and the (GR) was expressed from an expression vector parker et al. (1994). However, in the case of prolactin promoter fragments (eg, - 1960/+ 38, -44/ -423, -190/-423 and – 75 /+ 38) decreased in pitl induced DAT gene expression was seen when the (GR) was expressed but it was evident even in the absence of glucocorticoids.
Eljaafari, S., El-Fighi, A., & fahelbum, I. (2010). INHIBITION BY GLUCOCORTICOIDS OF PIT 1 INDUCED ACTIVATION OF TRANSCRIPTION FROM RPR1 PROMOTER FRAGMENTS IN NON PITUITARY CELLS. Biochemistry Letters, 6(1), 106-112. doi: 10.21608/blj.2010.64435
MLA
S. Eljaafari; A:B. El-Fighi; I.M. fahelbum. "INHIBITION BY GLUCOCORTICOIDS OF PIT 1 INDUCED ACTIVATION OF TRANSCRIPTION FROM RPR1 PROMOTER FRAGMENTS IN NON PITUITARY CELLS", Biochemistry Letters, 6, 1, 2010, 106-112. doi: 10.21608/blj.2010.64435
HARVARD
Eljaafari, S., El-Fighi, A., fahelbum, I. (2010). 'INHIBITION BY GLUCOCORTICOIDS OF PIT 1 INDUCED ACTIVATION OF TRANSCRIPTION FROM RPR1 PROMOTER FRAGMENTS IN NON PITUITARY CELLS', Biochemistry Letters, 6(1), pp. 106-112. doi: 10.21608/blj.2010.64435
VANCOUVER
Eljaafari, S., El-Fighi, A., fahelbum, I. INHIBITION BY GLUCOCORTICOIDS OF PIT 1 INDUCED ACTIVATION OF TRANSCRIPTION FROM RPR1 PROMOTER FRAGMENTS IN NON PITUITARY CELLS. Biochemistry Letters, 2010; 6(1): 106-112. doi: 10.21608/blj.2010.64435
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